| 引用本文:黄小平，李静娴，杨筱倩，丁煌，唐标，刘晓丹，邓常清.PC12细胞氧糖剥夺模型细胞自噬与损伤的关系及黄芪甲苷的保护作用研究[J].世界中医药,2016,(04):. |
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| PC12细胞氧糖剥夺模型细胞自噬与损伤的关系及黄芪甲苷的保护作用研究 |
| Relationship Between Autophagy and Damage in the Oxygen Glucose Deprivation Model of PC12 Cells and the Protective Effect of Astragaloside IV |
| 投稿时间：2016-03-24 |
| DOI：10.3969/j.issn.1673-7202.2016.04.008 |
| 中文关键词: PC12细胞 氧糖剥夺再复糖复氧 细胞自噬 细胞损伤 黄芪甲苷 保护作用 |
| English Keywords:PC12 cell Oxygen glucose deprivation/reoxygenation Autophagy Cell injury Astragaloside IV Protective effect |
| 基金项目:国家自然科学基金项目（编号：81473581；81573875）；湖南省高校创新平台开放基金资助项目（编号：11K050；14K068）；湖南省科技厅一般项目（编号：2014SK3001）；湖南省中医药管理局重点项目（编号：201301；201508）；中医内科重大疾病防治及成果转化省部共建教育部重点实验室开放课题（编号：ZYNK201405）；“中西医结合防治心脑血管疾病的相关基础研究”湖南省高校科技创新团队；“中医药防治心脑血管疾病基础研究”湖南省自然科学创新群体基金 |
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| 中文摘要: |
| 目的：研究PC12细胞OGD/R后自噬与损伤的经时性变化以及黄芪甲苷的干预效应。方法：探讨PC12细胞OGD/R后不同时间自噬的变化及与细胞损伤的关系，初步确定细胞发生自噬性损伤的时间点，再制作PC12细胞OGD/R自噬性损伤模型，研究黄芪甲苷抗自噬性损伤的作用。结果：复糖复氧6～36 h后，细胞存活率降低、LDH漏出率增加；用3-MA预处理后，可使复糖复氧6～12 h细胞存活率降低、LDH漏出率增加，复糖复氧后24～36 h相反。激光共聚焦和western-blot检测显示，复糖复氧后6 hLC3、LC3-Ⅱ/LC3-Ⅰ比值增加，至24 h达高峰；p62蛋白表达随再复糖复氧时间的延长逐渐降低。黄芪甲苷对OGD 2 h复糖复氧24 h的PC12细胞自噬具有显著抑制作用，且呈剂量依赖性。结论：PC12在在OGD复糖复氧6～12 h，自噬减轻神经细胞的损伤，而在24～36 h后加重细胞损伤；黄芪甲苷可通过抑制细胞过度自噬诱导的细胞损伤，从而发挥对受损细胞的神经保护作用。 |
| English Summary: |
| To explore the changes of autophagy and injury in different time periods after PC12 cell were induced by oxygen-glucose deprivation/reoxygenation (OGD/R), and the intervention effects of Astragaloside IV.Methods:Firstly, the changes of autophagy at different time periods and the relationship with cell damage after PC12 cell oxygen-glucose deprivation/reoxygenation (OGD/R) was probed to determine the point that autophagy contributed to cell injury, then the the injury model of PC12 OGD/R was established to investigate the effect of Astragaloside IV against autophagy injury.Results:After reoxygenation for 6～36 h, cell survival rate decreased, the leakage rate of LDH increased. Pretreated with 3-mA, cell survival rate decreased significantly, LDH leakage rate increased remarkably after reoxygenation for 6～12 h, while being opposite after reoxygenation 24～36 h. Confocal microscopy and western-blot showed that the autophagosome was formed after reoxygenation for 6 h and LC3-Ⅱ, LC3-Ⅱ/LC3-Ⅰwas increased, getting to the maximum at 24 h after reoxygenation. The expression of p62 protein decreased gradually along with time. Astragaloside IV could remarkably inhibit cell autophagy followed by PC12 cell OGD/R 24 h and presented dose dependence.Conclusion:Autophagy may reduce the damage of nerve cells at 6～12 h after reoxygenation, however, excessive autophagy aggravates the damage after reoxygenation for 24～36 h; Astragaloside IV exerts the protective effect from the injured cells through inhibiting the cell damage induced by excessive autophagy. |
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