世界中医药

目的：探讨心元胶囊对兔颈动脉斑块稳定性及VEGF表达的影响。方法：运用高脂高胆固醇饲料喂养12周制备日本大耳白兔动脉粥样硬化模型，分别以阿伐他汀钙（阳性对照）和心元胶囊灌胃给药治疗4周，检测各组血脂水平的变化，HE染色法观察动脉形态学，免疫组化检测CD105单克隆抗体在动脉斑块中的表达，RT-PCR检测VEGF mRNA的表达。结果：1）血脂水平变化，与空白组比较，其余各组血清中TC、TG、LDL-C均显著升高，HDL-C显著降低，差异具有统计学意义（P<0.05，P<0.01）；与模型组比较，药物干预组的血脂水平明显改善；2）形态学观察，空白组动脉厚薄均匀，结构完整，内膜、中膜及外膜形状规则，未见异常；模型组可见典型不稳定斑块，动脉内膜明显增厚，管腔变窄；3）D105免疫组化显示：空白组几乎没有表达，模型组的表达最强（P<0.01），与模型组比较，药物干预组的阳性表达显著减少（P<0.05），药物干预组阳性染色面积显著降低。4）VEGF mRNA的表达显示，与空白组比较，模型组颈总动脉硬化斑块处VEGF mRNA表达显著增强(P<0.01）；与模型组比较，药物干预组均能显著下调粥样硬化处VEGF mRNA的表达(P<0.05)，心元胶囊组的表达略高于阿伐他汀钙组。结论：心元胶囊同阿伐他汀钙一样能有效改善动脉粥样硬化斑块内CD105的表达，下调VEGF mRNA的表达，从而达到保持斑块稳定甚至减小斑块的目的。

English Summary:

To investigate the effects of Xinyuan Capsule on stability of rabbit atherosclerotic plaque and its VEGF expression. Methods: Japanese big ear rabbit were fed high fat and high cholesterol diet for 12 weeks to make atherosclerosis model. Rabbits were respectively treated by atorvastatin calcium (positive control) and Xinyuan Capsule for 4 weeks to detect the change of blood lipid level in each group. Arterial morphology was observed by HE staining, CD105 monoclonal antibody in artery plaques was detected by immunohisto-chemical staining and the expression of VEGF mRNA was detected by RT-PCR. Results: 1) There was changes of blood lipid levels compared with the blank group, plasma TC,TG,and LDL-C in all the rest groups were significantly increased and HDL-C decreased, which showed significant difference between each group(P<0.05,P<0.01);blood lipid level of drug intervention group improved obviously compared with the model group. 2)Artery of rabbits in the blank group was even in thickness, complete in structure,regular in intima and media and adventitia shape without any abnormality, while typical unstable plaque, obvious arterial intimal thickening,channel narrowing showed in the model group. 3)The CD105 immunohisto-chemistry showed merely no expression in the blank group and the expression of the model group was the strongest (P<0.01), and the positive expression of drug intervention group was significantly decreased (P<0.05) and positive staining area decreased significantly, compared with the model group. 4) It was showed that the VEGF in mRNA expression of the total atherosclerotic plaque in the model group was significantly enhanced compared with the blank group (P<0.01), while that of the drug intervention group was significantly decreased compared with the model group (P<0.05). The expression in Xinyuan Capsule group was higher than that of the atorvastatin calcium group. Conclusion: The Xinyuan capsule, with the similar effect compared with atorvastatin calcium,may effectively improve the expression of CD105 in atherosclerotic plaques and decrease the expression of VEGF mRNA,to maintain the plaque stability and even reduce plaque.

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