| 引用本文:张瑞瑞1，刘艳萍4，聂西周1，杨东花2，刘峰2,3.射干抗病毒注射液致类过敏反应初步研究[J].世界中医药,2016,(04):. |
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| 射干抗病毒注射液致类过敏反应初步研究 |
| Preliminary Study on the Pseudoallergic Reaction of Shegan Kangbingdu Injection |
| 投稿时间：2015-08-24 |
| DOI：10.3969/j.issn.1673-7202.2016.04.031 |
| 中文关键词: 射干抗病毒注射液 类过敏反应 RBL-2H3细胞 |
| English Keywords:SG Pseudo-allergic reaction RBL-2H3 cell |
| 基金项目:陕西省重点科技创新团队计划项目(编号：2015KCT-19) |
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| 中文摘要: |
| 目的：探究射干抗病毒注射液的致类过敏反应及其可能的作用机制。方法：通过体外动物类过敏试验，探究射干抗病毒注射液对小鼠耳廓血管通透性的影响；细胞水平上，采用MTT比色法筛选出射干抗病毒注射液对RBL-2H3细胞的安全作用浓度，并在安全浓度之下，以不同浓度，用中性红染色观察其脱颗粒状态，检测射干抗病毒注射液对细胞内颗粒物质β-氨基己糖苷酶释放量的影响，Fluo-3荧光标记细胞内Ca2+检测细胞内钙离子变化，综合考察射干抗病毒注射液的体外致类过敏性。结果：射干抗病毒注射液可引起小鼠耳廓通透性增强，引发类过敏反应；并可诱导RBL-2H3细胞发生脱颗粒反应，使胞内钙离子浓度升高，且均具有剂量依赖性。结论：射干抗病毒注射液具有引发类过敏反应的风险，其机制可能与引起胞内Ca2+浓度增加有关。 |
| English Summary: |
| To explore the effect of Shegan Kangbingdu injection(SG) on inducing pseudo-allergic reaction. Methods: Animal allergy experiment was taken to explore the allergic effects on mice. MTT was used to confirm the concentration on RBL-2H3 cells. Cell degranulation effect was verified by neutral red staining experiment, and β-hexosaminidase release rate test. Fluo-3 was used to detect intracellular Ca2+ changes. The allergic reactions of SG were thus detected comprehensively. Results: SG enhanced mice auricle permeability and thus induced mice allergy; different concentrations of SG induced RBL-2H3 cell degranulation, the intracellular calciumion concentration has obviously increased due to dose-dependence Conclusion: SG can induce mice allergy, and this might related to intracellular Ca2+ increase. |
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