| 引用本文:孙丽，刘英北，王岭，陈晶，李海燕，王超，李艳.黄芪注射液对大鼠脑缺血再灌注损伤后BDNF表达的影响[J].世界中医药,2016,(04):. |
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| 黄芪注射液对大鼠脑缺血再灌注损伤后BDNF表达的影响 |
| Effect of Astragalus Injection on Expression of BDNF in Cerebral Ischemia Reperfusion Injury in Rats |
| 投稿时间：2015-04-28 |
| DOI：10.3969/j.issn.1673-7202.2016.04.032 |
| 中文关键词: 脑缺血 再灌注损伤 黄芪注射液 BDNF 大鼠 |
| English Keywords:Cerebral ischemia Reperfusion injury Astragalus injection BDNF Rats |
| 基金项目:山东省医药卫生科技发展计划（编号:2011HW034） |
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| 中文摘要: |
| 目的：探讨黄芪注射液对大鼠脑缺血/再灌注损伤后脑源性神经营养因子（BDNF）表达的影响。方法：应用线栓法建立大鼠大脑中动脉缺血再灌注（MCAO/R）模型，经腹腔注射黄芪注射液（6 mL/kg）干预治疗。Longa法评分评价大鼠神经行为功能，蛋白印迹法（Western-blot）定量检测BDNF蛋白的表达，荧光定量PCR（Real-Time PCR）检测BDNF基因的表达。结果：经黄芪注射液治疗后，大鼠大脑海马区BDNF蛋白和BDNF mRNA表达较对照组明显增加，动物神经行为功能显著改善。结论：黄芪注射液可通过上调BDNF的表达而促进受损神经的修复，刺激神经再生，改善动物的神经行为功能。 |
| English Summary: |
| To investigate the effects of astragalus injection on expression of brain derived neurotrophic factor (BDNF) in cerebral ischemia reperfusion injury in rats. Methods: The middle cerebral artery occlusion reperfusion (MCAO/R) rat models were established by inserting filament suture from right external-internal carotid artery and treated by intraperitoneal astragalus injection (6 mL/kg). The neurobehavioral function of rats was evaluated by Longa’s test, and neuronal apoptosis was detected by TUNEL staining with Western-blot as the quantity analysis method to detect the expressions of brain derived neurotrophic factor (BDNF). The expression of BDNF mRNA was determined by Real-Time PCR. Results: After the treatment with astragalus injection, the expression of BDNF and BDNF mRNA in rats hippocampus increased significantly compared with those of the control group. Neuronal apoptosis was significantly reduced and the animal neurobehavioral function improved significantly. Conclusion: Astragalus injection may inhibit apoptosis, promote the nerve repair, stimulate the nerve regeneration and improve the nervous behavioral function by up-regulated the expressions of BDNF. |
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