| 引用本文:张赛1，杜庆红1，李卫红1，马家宝1，姜昭妍1，胡艳红1，郭晓谨2，万亮琴1，李芳赫1.三七总皂苷对拟缺血脑微血管内皮细胞RIG-I表达的影响[J].世界中医药,2016,(04):. |
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| 三七总皂苷对拟缺血脑微血管内皮细胞RIG-I表达的影响 |
| The effect of Panax notoginseng saponins on the expression of RIG-I in mimic ischemic cerebral microvascular endothelial cells |
| 投稿时间：2015-12-25 |
| DOI：10.3969/j.issn.1673-7202.2016.04.035 |
| 中文关键词: 三七总皂苷 脑微血管内皮细胞 维甲酸诱导基因-I |
| English Keywords:Panax notoginseseng saponins Cerebral microvascular endothelial cells Retinoic acid-inducible gene-I |
| 基金项目:国家自然科学基金项目(编号:81273885);北京中医药大学研究生自主课题项目(编号:2015-JYB-XS043);北京市自然科学基金项目(编号:7144223) |
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| 中文摘要: |
| 目的:研究三七总皂苷对拟缺血损伤的脑微血管内皮细胞维甲酸诱导基因-I(Retinoic Acid-inducible Gene-I,RIG-I)的影响。方法:采用原代培养大鼠脑微血管内皮细胞(Brain Microvascular Endothelial Cell,BMEC),氧糖剥夺法制备拟缺血损伤模型,首先用不同浓度梯度的三七总皂苷进行干预,筛选出三七总皂苷的最佳用药浓度,然后分别采用荧光定量PCR和免疫蛋白印迹法检测各组细胞中RIG-I mRNA及蛋白的表达水平。结果:三七总皂苷在22 μg/mL浓度时能够显著提高拟缺血损伤脑微血管内皮细胞的活性,同时能够从基因和蛋白水平下调RIG-I的表达。结论:三七总皂苷对拟缺血损伤脑微血管内皮细胞具有保护作用,提示三七总皂苷在缺血性脑损伤中使RIG-I的表达下调可能是其发挥抗炎作用的内在分子机制之一。 |
| English Summary: |
| To study the effect of Panax notoginseng saponins(PNS)on the expression of RIG-I in mimic ischemic cerebral microvascular endothelial cells(BMECs).Methods:Rat BMECs were cultured in oxygen-glucose-deprived(OGD) conditions to mimic cerebral ischemia in vitro.Firstly，the optimal concentration of PNS was determined by various concentration?gradients of PNS being administrated on OGD-induced BMECs.Then,the expression levels of RIG-I mRNA and protein in each group cells were detected by Q-PCR and Western blot method.Results:PNS can significantly improve the cells activity at 22μg/mL concentration，and decrease the expression of RIG-I in gene and protein levels in the OGD-induced BMECs.Conclusion:PNS has a protective effect on mimic ischemic BMECs，the mechanism may be related to the down-regulation of RIG-I expression，which may be one of the mechanisms of anti-inflammatory effects of PNS in cerebral ischemic injury. |
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