| 引用本文:朱俊访，李博.响应面法优化淡竹叶总黄酮的大孔树脂纯化工艺[J].世界中医药,2016,(04):. |
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| 响应面法优化淡竹叶总黄酮的大孔树脂纯化工艺 |
| Optimization of Purification Technology Using Macroporous Resin for Total Flavonoids from Lophatherum Gracile with Response Surface Method |
| 投稿时间：2015-10-15 |
| DOI：10.3969/j.issn.1673-7202.2016.04.039 |
| 中文关键词: 淡竹叶 总黄酮 大孔树脂 响应面法 |
| English Keywords:Lophatherum gracile Total flavonoids Macroporous resin Response surface method |
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| 摘要点击次数: 843 |
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| 中文摘要: |
| 目的：探讨响应面法优化淡竹叶总黄酮大孔树脂纯化工艺。方法：比较四种大孔树脂对淡竹叶总黄酮的吸附和解吸性质，筛选合适的树脂，采用响应面法优化纯化工艺。结果：通过实验数据分析，较佳纯化工艺条件为上样液质量浓度0.12 g/mL，乙醇体积分数75%，洗脱液用量6BV。结论：通过响应面法优选的淡竹叶总黄酮大孔树脂纯化工艺稳定可行，预测性好。 |
| English Summary: |
| To optimize purification technology using macroporous resin for total flavonoids from lophatherum gracile with response surface method. Methods: Compare the adsorption and desorption properties of four macroporous resin on total flavonoids of lophatherum gracile. Select the appropriate resin to optimize purification process with response surface method. Results: According to the analysis of experimental data, it was better to optimize purification process with the mass concentration 0.12 g/mL of sample solution, volume fraction 75% of ethanol and eluent dosage 6BV. Conclusion: It was stable and feasible with good predictability to optimize the macroporous resin purification process for total flavonoids from lophatherum gracile with response surface method. |
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