| 引用本文:李朝霞1，欧阳俊摇1，王雅丽1，赵晖1，沈素2，王丽丽2，李任2.蛭丹化瘀组方对内毒素诱导肺损伤大鼠CGRP、ET-1的影响[J].世界中医药,2016,(05):. |
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| 蛭丹化瘀组方对内毒素诱导肺损伤大鼠CGRP、ET-1的影响 |
| Effects of Zhi Dan Hua Yu prescription on CGRP and ET-1 of lung injury rats induced by LPS |
| 投稿时间：2016-02-29 |
| DOI：10.3969/j.issn.1673-7202.2016.05.031 |
| 中文关键词: 蛭丹化瘀组方 肺损伤 降钙素基因相关肽 内皮素-1 |
| English Keywords:Zhi Dan Hua Yu prescription Lung injury CGRP ET-1 |
| 基金项目:北京市科学技术委员会“十病十药”研发项目(编号:Z141100002214019)——“蛭丹化瘀组方用于治疗小儿病毒性肺炎的成药性研究” |
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| 中文摘要: |
| 目的:探讨蛭丹化瘀组方对内毒素诱导肺损伤大鼠血浆降钙素基因相关肽(CGRP)、内皮素-1(ET-1)、肿瘤坏死因子(TNF-α)的影响。方法:SD大鼠84只,雌雄各半,随机分为对照组、模型组、蛭丹化瘀组方3个剂量(33、16.5、8.25 g/kg)。大鼠气管内滴注LPS复制急性肺损伤大鼠模型,称重法检测大鼠肺含水量,放免法检测各组大鼠血浆ET、CGRP、TNF-α的变化。结果:模型组大鼠肺含水量较正常组明显升高(P<0.01),蛭丹化瘀组方(33 g/kg、16.5 g/kg)(P<0.01、P<0.05)以及阳性药地塞米松(P<0.01)可降低内毒素诱导肺损伤大鼠的肺含水量;与正常组比较,模型组大鼠血浆中内皮素(ET)、降钙素基因肽(CGRP)明显增高(P<0.01);蛭丹化瘀组方(16.5 g/kg、8.25 g/kg)和阳性药地塞米松可明显降低大鼠血浆ET含量,差异较模型组明显(P<0.01);蛭丹化瘀组方33 g/kg可明显增加血浆CGRP含量;与正常组比较,模型组大鼠血浆肿瘤坏死因子(TNF-α)明显增高(P<0.01);阳性药地塞米松及蛭丹化瘀组方(33 g/kg、16.5 g/kg、8.25 g/kg)均可明显降低大鼠血浆TNF-α含量(P<0.01)。结论:蛭丹化瘀组方方通过升高内毒素诱导肺损伤大鼠CGRP的含量,降低ET-1及TNF-α含量发挥对肺损伤大鼠的保护作用。 |
| English Summary: |
| To observe the effect of Zhi Dan Hua Yu prescriptionon CGRP、ET-1 and TNF-αof lung injury rats induced byendotoxin.Methods:A total of 84 SD rats withhalf males and half females were divided into control group,model group,Zhi Dan Hua Yu prescription group(33 g/kg,16.5 g/kg,8.25 g/kg)and dexamethasone group by random,detecting the changes ofwatercontent inlung injury rats’ lung；utilizing radioimmunoassay to detecting changes of ET and CGRP in rat plasma.Results:Compared to control group，lung water content in model group rats were significantly increased(P<0.01),Zhi Dan Hua Yu prescription(33 g/kg,16.5 g/kg)and dexamethasone obviously reduced water content in lunginjury rats.The endothelin(ET)and calcitonin gene peptide(CGRP)in rat plasmaof model group were significantly increased(P<0.01)compared to that of control group;dexamethasone reduced ET content(P<0.01)and Zhi Dan Hua Yu prescription(16.5 g/kg,8.25 g/kg)reduced ET contant apparently(P<0.01); CGRP contant in rat plasma of Zhi Dan Hua Yuprescription(33 g/kg)were increased significantly(P<0.01); The content of tumor necrosis factor-α(TNF-α)was increased apparently(P<0.01)in comparison with that of control group; dexamethasone and Zhi Dan Hua Yu prescription(33 g/kg,16.5 g/kg,8.25 g/kg)reduced TNF-αin rat plasma obviously(P<0.01).Conclusion:Zhi Dan Hua Yu prescriptionprotect endotoxin-induced lung injury rats by increase CGRP content and reduce ET-1 and TNF-αcontent in rat plasma. |
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