To observe the correlation between respiratory function and urine volume change and to explore the influence of “lung governing respiration” on “regulating waterway”. Methods:The effects of changes in urine volume, as the main index, respiratory function changes of asthma mice model and chronic obstructive lung disease (COPD) model and positive pressure expanding lung in rabbit pulmonary ventilatory activity changes on “regulating waterway” were observed. Urine volume of each animal was measured set for a specific time period and urine volume of model groups was compared with that of the control group. Results:The urine volumes of three experimental animal models were lower than those in the control group. 1) Five-hour total urine volume of asthma mice model of was less than that of the control group (P<0.05). 2) Total volume urine of COPD rat model at 6 h, 12 h, 18 h and 24 h separately were higher than those of the control group (P<0.05). 3) Urine drop number of positive pressure lung-expanding rabbits reduced compared to that during their 10 min normal breathing (P<0.05) and the one after 10 min expending was also decreased compared with that of the control group (P<0.05). Conclusion:The experimental basis of the effect of “lung governing respiration” on “regulating waterway” was obtained to further explore the related molecular signaling pathways, providing foundation for modern biological mechanism.