| 引用本文:魏艺聪，陈建雄，黄泽豪，卢伟，车苏容，梁一池.基于matK序列的积雪草与其易混品的分子鉴定方法分析[J].世界中医药,2016,(05):. |
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| 基于matK序列的积雪草与其易混品的分子鉴定方法分析 |
| Identification of Centella asiatica and adulterated Species Based on GenBank Nucleotide Database matK Sequence |
| 投稿时间：2016-01-22 |
| DOI：10.3969/j.issn.1673-7202.2016.05.040 |
| 中文关键词: 积雪草 混淆品 matK序列 分子鉴别 |
| English Keywords:Centella asiatica Adulterated Species matK sequence Identification |
| 基金项目:中医药公益性行业专项（编号：201407002） |
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| 中文摘要: |
| 目的：积雪草容易与多种混淆品相互混淆，本研究拟基于matK序列探讨积雪草与其混淆品鉴定的新方法。方法：从GenBank核酸数据库下载积雪草、过路黄、连钱草和乌蔹莓的matK序列，应用ClustalX 2.1软件进行SNP鉴别位点分析，应用MEGA5.0软件计算种内种间（K2P）遗传距离和构建邻接（NJ）系统聚类树。结果：获得积雪草与过路黄各5个matK序列及连钱草和乌蔹莓各1个matK序列，分析显示积雪草与其混伪品的matK序列存在较大差异，具有近百个SNP位点，且其中多是积雪草特有的，可用于分子鉴定。且积雪草最大种内K2P遗传距离0.001，远远小于其与混伪品的种间K2P遗传距离0.242~0.293，构建的系统发育树显示积雪草与其混淆品可明显分开。结论：综合以上分析，matK序列为鉴定积雪草及其混淆品提供了新的分子鉴定方法。 |
| English Summary: |
| To identify Centella asiatica and adulterated Species by using GenBank nucleicacid database matK sequences.Methods:Based on GenBank Nucleotide Database，matK sequences of Centella asiatica and adulterated Species were downloaded.The SNP sites were checked by ClustalX 2.1 software，K2P Distances were calculated using MEGA 5.0 software and neighbor-ioining（NJ）phylogenetic tree was established.Results:There many specific SNP sites can be used to molecular authentication of Centella asiatica and adulterated Species.K2P genetic distance between Centella asiatica and adulterated Species is between 0.242～0.293，which was far more than intraspecific genetic distance of Centella asiatica（0.001）and adulterated Species（0.004）.Results of NJ phylogenetic tree showed that Centella asiatica can be separated from adulterated Species and classified into a separate one.Conclusion:matK sequence is suitable for the identification of Centella asiatica and adulterated Species and provides reference for quality and safety identification of Centella asiatica and adulterated Species. |
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