To establish an HPLC-ELSD method for determination of cholic acid in Xiaoer Qingrening Granule. Methods:Samples were prepared by ultrasonic extraction of methanol for 30 min. Venusil XBP C18 (L) (4.6 mm × 250 mm, 5 μm) column was used with methanol-0.1% phosphoric acid solution (75:25, v/v) with the mobile phase at 1.0 mL/min. The temperature of column was set at 25 ℃ and drift tube was 40 ℃. The flow rate of gas was maintained at 2.0 L/min. Results:The linearity was obtainedfrom 0.502~2.51 μg (r=0.999 4). The average recovery was 100.532 1% with RSD=1.969 6%. Conclusion:The method is specific, and can be used for quality control of Xiaoer Qingrening Granule.