| 引用本文:胡琦，谭小宁，余娜，朱沛，陈思勤，朱克俭.臭牡丹总黄酮介导Wnt/β-catenin信号转导诱导人肝癌细胞HepG2的凋亡[J].世界中医药,2016,(06):. |
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| 臭牡丹总黄酮介导Wnt/β-catenin信号转导诱导人肝癌细胞HepG2的凋亡 |
| Research on the Medicate Effect of Clerodendron Bungei Flavornoids on Wnt/β-catenin in Inducing the Apoptosis of HepG2 |
| 投稿时间：2016-05-25 |
| DOI：10.3969/j.issn.1673-7202.2016.06.004 |
| 中文关键词: 臭牡丹总黄酮 肝癌 Wnt/β-catenin信号通路 凋亡 |
| English Keywords:Clerodendron bungei flavornoids Liver cancer Wnt/β-catenin pathway Apoptosis |
| 基金项目:国家自然科学基金项目（编号：81503452） |
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| 中文摘要: |
| 目的：探讨臭牡丹总黄酮诱导肝癌细胞凋亡的作用及机制。方法：体外培养HepG2细胞，流式细胞术检测臭牡丹总黄酮对细胞凋亡的影响；RT-PCR法检测臭牡丹总黄酮作用于HepG2细胞48 h后对β-catenin、Tcf-4、CD44v6 mRNA表达的影响。结果：流式细胞术结果显示：随着臭牡丹总黄酮剂量的增加，细胞凋亡比例亦相应增加。PT-PCR结果显示不同剂量臭牡丹总黄酮作用下β-catenin、Tcf-4、CD44v6 mRNA的表达均降低，与对照组相比具有统计学意义。结论：臭牡丹总黄酮诱导HepG2凋亡，能下调β-catenin、Tcf-4、CD44v6基因的表达，其作用机制可能与调控Wnt/β-catenin信号通路并抑制其关键基因有关。 |
| English Summary: |
| To discuss the apoptotic effect of Clerodendron bungei flavornoids on liver tumor cells and the possible mechanism.Methods:To culture HepG2 cells in virto,and investigate the apototic effect of clerodendron bungei flavornoidson by flow cytometry.The mRNA expression levels of proteins β-catenin,Tcf-4 and CD44v6 of HepG2 tumor cells were detected by RT-PCR after 48h treatment of flavornoids.Results:Flow cytometry indicates that along with the rising dose of Clerodendron bungei flavornoids,the apoptosis rate of HepG2 cancer cells begin to grow.After 48h treatment of flavornoids,the mRNA expression levels of proteins β-catenin,Tcf-4 and CD44v6 of HepG2 tumor cells were down-regulated significantly,and compared with the control group,significant difference exist.Conclusion:Clerodendron bungei flavornoids can induce the apoptosis of HepG2 tumor cells and decrease the mRNA expression levels of proteins β-catenin,Tcf-4 and CD44v6.This might relate to its regulating effect on Wnt/β-catenin pathway and inhibit the key gene’s expression. |
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