| 引用本文:马进1，甘雨1，袁媛1，黄赫1，乔铁2，崔英海1，刘曼玉2，褚丽2，田淼淼2.川芎赤芍干预脑缺血再灌注大鼠血管新生的实验研究[J].世界中医药,2016,(06):. |
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| 川芎赤芍干预脑缺血再灌注大鼠血管新生的实验研究 |
| Study of Sichuan Lovage Rhizome and Red Peony Root on Intervening Angiogenesis of Rats with Cerebral Ischemia-reperfusion |
| 投稿时间：2016-03-29 |
| DOI：10.3969/j.issn.1673-7202.2016.06.033 |
| 中文关键词: 脑缺血再灌注 血管新生 Ang-1 HIF-1a |
| English Keywords:Cerebral ischemia reperfusion Angiogenesis Ang-1 HIF-1a |
| 基金项目:辽宁省教育厅科学技术计划项目(编号:L2013362);辽宁中医药大学杏林学者青蓝工程杰出青年项目 |
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| 中文摘要: |
| 目的:观察川芎赤芍对大鼠脑梗死再灌注后血管生成素-1(Ang-1)、缺氧诱导因子-1a(HIF-1a)的影响。方法:线栓法制备大鼠大脑中动脉缺血再灌注模型,将SD大鼠随机分为空白组、模型组、川芎赤芍低、高剂量组、银杏叶片组。ELISA法测定大鼠血清Ang-1,实时荧光定量PCR方法检测缺血脑组织HIF-1a mRNA表达。结果:大鼠血清Ang-1测定:给药3 d、7 d、14 d模型组与空白组相比升高,具有统计学意义(P<0.05);给药3 d、7 d、14 d模型组与川芎赤芍低剂量组、高剂量组、银杏叶组相比水平低,具有统计学意义(P<0.05);实时荧光定量PCR检测HIF-1a mRNA:给药3 d、7 d、14 d模型组与空白组相比表达升高,具有统计学意义(P<0.05);给药3 d、7 d、14 d模型组与川芎赤芍低剂量组、高剂量组、银杏叶组相比表达水平低,具有统计学意义(P<0.05)。结论:川芎赤芍可升高缺血再灌注大鼠血清中Ang-1的水平及缺血脑组织中HIF-1a mRNA的表达,提示川芎赤芍治疗脑梗死机制可能与促进血管新生有关。 |
| English Summary: |
| To observe the effect of Sichuan Lovage Rhizome and Red Peony Root on angiogenin-1 (Ang-1),ischemia induced factor-1 a (HIF-1 a) on rats with cerebral ischemia-reperfusion.Methods:The model of rat with middle cerebral artery ischemia-reperfusion was established with suture-occluded method.SD rats were randomly divided into control group,model group and Sichuan Lovage Rhizome and Red Peony Root low and high dose groups,Ginkgo group.Ang-1 in rat serum was tested with ELISA method.HIF-1a mRNA expression in ischemia brain tissue was detected with real-time fluorescent quantitative polymerase chain reaction (rt-PCR).Results:Ang-1 in rat serum in model groups increased on days 3,7 and 14 compared with the control group (P<0.05).HIF-1a expression with Rt-PCR increased in model groups on day 3,7 and 14 compared with control group (P<0.05).HIF-1a expression in model group on day 3,7 and 14 was lower than Sichuan Lovage Rhizome and Red Peony Root low and high dose groups,Ginkgo group (P<0.05).Conclusion:Sichuan Lovage Rhizome and Red Peony Root could enhance Ang-1 in rats with cerebral ischemia-reperfusion and HIF-1a mRNA expression in ischemic brain tissue,suggesting that the mechanism of Sichuan Lovage Rhizome and Red Peony Root on treating cerebral ischemia may be related with promoting angiogenesis. |
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