世界中医药

目的：探讨大柴胡汤加减方对肝功能和凝血机制的作用。方法：50只大鼠随机分为3组，正常对照组（A组）10只，模型组（B组）20只，大柴胡汤组（C组）20只。C组大鼠于造模前2 d分别灌胃大柴胡汤10 mL/（kg·d），A、B组予同等剂量0.9%氯化钠注射液灌胃，B、C组大鼠均采用皮下注射硫代乙酰胺0.2 g/kg，24 h后相同剂量重复皮注1次，48 h后完成急性肝衰竭动物模型，首次注射硫代乙酰胺的大鼠，予皮下注射10%葡萄糖注射液0.25 g/kg。观察造模完成后继续予大柴胡汤灌胃3 d，A组大鼠予同等剂量0.9%氯化钠注射液灌胃，3 d后取血处死所有大鼠，保存大鼠血清和病理组织。观察3组大鼠病理组织、肝功能（ALT、AST、ALB、CHE）、凝血功能（PT）指标的情况。结果：与模型组比较，大柴胡汤组大鼠组织肝衰竭减轻；模型组大鼠ALT、AST、TBiL与正常组大鼠比较，明显升高，差异具有统计学意义（P<0.05），模型组大鼠ALB、CHE与正常组大鼠比较，显著降低，差异具有统计学意义（P<0.05），大柴胡汤组大鼠ALT、AST、TBiL与模型组大鼠比较，明显降低，差异具有统计学意义（P<0.05），大柴胡汤组大鼠ALB、CHE与正常组大鼠比较，显著升高，差异具有统计学意义（P<0.05）；模型组大鼠PT与正常组大鼠比较，明显升高，差异具有统计学意义（P<0.05），大柴胡汤组大鼠PT与模型组大鼠比较，明显降低，差异具有统计学意义（P<0.05）。结论：大柴胡汤加减方对肝功能和凝血机制具有保护作用。

English Summary:

To evaluate the Modified Major Bupleurum Decoction’s effect on liver function and blood coagulation mechanism.Methods:A total of 50 rats were randomly divided into three groups:normal group (A group) with 10 rats,model group (B group) with 20 rats and Bupleurum Decoction group (C group) with 20 rats.C group were given 10 mL/(kg.d) oral Bupleurum Decoction 2 days before modeling.A and B group were given the same dose of 0.9%NaCl.B and C group rats were given subcutaneous injection of thioacetamide 0.2 g/kg per 24h for two days and then the acute liver failure models were established.For rats injected with thioacetamide for the first time,10%GS 0.25 g/kg were also administrated.After the model was established,Major Bupleurum Decoction lavage were given for three days,and A group rats were given the same dose of 0.9%NaCl.Three days later,all the rats were killed to take blood sample,and rats serum and tissue pathology were kept after 3 days.Pathological tissue,liver function indexes (ALT,AST,ALB,CHE),blood coagulation function indexes (PT) were compared among the three groups.Results:Compared with model group,the tissue of Major Bupleurum Decoction group was better; ALT,AST,TBiL of model group leveled up significantly compared with normal group rats(P<0.05),ALB and CHE of model group rats decreased significantly compared with normal group (P<0.05).Compared with model group,ALT,AST,TBiL of Major Bupleurum Decoction group decreased significantly(P<0.05),ALB、CHE of Major Bupleurum Decoction group increased significantly (P<0.05); PT of model group increased significantly compared with the normal group(P<0.05),PT of Major Bupleurum Decoction group decreased significantly compared with model group(P<0.05).Conclusion:Modified Major Bupleurum Decoction plays a protective role in liver function and blood coagulation mechanism.

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