引用本文:王萍1,王宇鹤2,贺新怀1,辛爱洁1,刘亚倩1.不同纯化方法对丹参水提取液中丹酚酸B的含量影响[J].世界中医药,2016,(06):. |
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不同纯化方法对丹参水提取液中丹酚酸B的含量影响 |
Effects of Different Purification Methods on Content of Salvianolic Acid B in Water Extract from Salvia Miltiorrhiza Bge |
投稿时间:2015-10-11 |
DOI:10.3969/j.issn.1673-7202.2016.06.039 |
中文关键词: 丹参 丹酚酸B 乙醇沉淀法 高速离心法 大孔树脂吸附法 |
English Keywords:Salvia Miltiorrhiza Bge Salvianolic Acid B Ethanol precipitation High speed centrifugation Macroporous absorption resin |
基金项目:陕西省中医药管理局科研项目(编号:13-zy024);陕西省教育厅科研项目(编号:14JK2017) |
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中文摘要: |
目的:考察不同纯化方法对丹参水提液中丹酚酸B含量的影响,以优选出丹参水提取液的最佳纯化方法。方法:以丹酚酸B含量和浸膏得率为考察指标,对乙醇沉淀法、高速离心法和大孔树脂吸附法3种纯化方法进行了比较。结果:大孔树脂吸附法处理后所得到的丹酚酸B的含量最高,浸膏得率最低;乙醇沉淀法处理后丹酚酸B的含量最低,浸膏得率居中;高速离心法处理后丹酚酸B的含量居中,但浸膏得率最高。结论:大孔树脂吸附法为丹参水提液的最佳纯化方法。 |
English Summary: |
To explore the best purification method by observing effects of different purification ways on the extractive rate of salvianolic acid B in Salvia Miltiorrhiza Bge water-extraction. Methods: Salvianolic acid B content and dry extract yield rate were used as indexes for assessing the best purification method among ethanol precipitation, high speed centrifugation and macroporous resin absorption. Results: Among all three methods, salvianolic acid B content by macroporous absorption resin was the highest, with dry extract yield rate being the lowest; salvianolic acid B content by ethanol precipitation was the lowest, with dry extract yield rate being the medium level; and salvianolic acid B content by high speed centrifugation was at the medium level, with dry extract yield rate being the highest. Conclusion: Water extraction of Salvia miltiorrhiza Bge by macroporous absorption resin is considered as the best purification method. |
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