| 引用本文:王萍1，王宇鹤2，贺新怀1，辛爱洁1，刘亚倩1.不同纯化方法对丹参水提取液中丹酚酸B的含量影响[J].世界中医药,2016,(06):. |
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| 不同纯化方法对丹参水提取液中丹酚酸B的含量影响 |
| Effects of Different Purification Methods on Content of Salvianolic Acid B in Water Extract from Salvia Miltiorrhiza Bge |
| 投稿时间：2015-10-11 |
| DOI：10.3969/j.issn.1673-7202.2016.06.039 |
| 中文关键词: 丹参 丹酚酸B 乙醇沉淀法 高速离心法 大孔树脂吸附法 |
| English Keywords:Salvia Miltiorrhiza Bge Salvianolic Acid B Ethanol precipitation High speed centrifugation Macroporous absorption resin |
| 基金项目:陕西省中医药管理局科研项目（编号：13-zy024）；陕西省教育厅科研项目（编号：14JK2017） |
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| 中文摘要: |
| 目的：考察不同纯化方法对丹参水提液中丹酚酸B含量的影响，以优选出丹参水提取液的最佳纯化方法。方法：以丹酚酸B含量和浸膏得率为考察指标，对乙醇沉淀法、高速离心法和大孔树脂吸附法3种纯化方法进行了比较。结果：大孔树脂吸附法处理后所得到的丹酚酸B的含量最高，浸膏得率最低；乙醇沉淀法处理后丹酚酸B的含量最低，浸膏得率居中；高速离心法处理后丹酚酸B的含量居中，但浸膏得率最高。结论：大孔树脂吸附法为丹参水提液的最佳纯化方法。 |
| English Summary: |
| To explore the best purification method by observing effects of different purification ways on the extractive rate of salvianolic acid B in Salvia Miltiorrhiza Bge water-extraction. Methods: Salvianolic acid B content and dry extract yield rate were used as indexes for assessing the best purification method among ethanol precipitation, high speed centrifugation and macroporous resin absorption. Results: Among all three methods, salvianolic acid B content by macroporous absorption resin was the highest, with dry extract yield rate being the lowest; salvianolic acid B content by ethanol precipitation was the lowest, with dry extract yield rate being the medium level; and salvianolic acid B content by high speed centrifugation was at the medium level, with dry extract yield rate being the highest. Conclusion: Water extraction of Salvia miltiorrhiza Bge by macroporous absorption resin is considered as the best purification method. |
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