| 引用本文:崔恩忠，唐安福，贡磊，汤淏，王曙东.HPLC法测定健肾丸中腺苷的含量[J].世界中医药,2016,(06):. |
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| HPLC法测定健肾丸中腺苷的含量 |
| HPLC Determination of Adenosine in Jianshen Pill |
| 投稿时间：2015-07-07 |
| DOI：10.3969/j.issn.1673-7202.2016.06.042 |
| 中文关键词: 健肾丸 腺苷 高效液相色谱法 |
| English Keywords:Jianshen Pill Acetonitrile HPLC |
| 基金项目:全军医疗机构制剂质量标准提升专项课题重点项目(编号:13ZJZ13)——“肾病系列非标准制剂质量标准提升的研究” |
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| 中文摘要: |
| 目的:建立健肾丸中腺苷含量的测定方法,为提高该制剂的质量标准提供研究依据。方法:采用高效液相色谱法,流动相:乙腈-水(6：94);色谱柱:Agilent TC-C18(2)柱(4.6 mm×250 mm,5 μm);流速为1.0 mL/min,检测波长为260 nm,柱温为25 ℃;进样量:10 μL。结果:腺苷在1.25~20.00 μg/mL范围内线性关系良好,平均回收率为101.04%,相对标准偏差(RSD)为1.93%。结论:该方法简便、可靠、重现性好,可用于健肾丸的质量控制。 |
| English Summary: |
| To provide scientific proof for promoting quality standard of Jianshen Pill by determining the level of adenosine in it.Methods:HPLC(High Performance Liquid Chromatography) method was used with Agilent TC-C18(2) column ( 250 mm×4.6 mm,5 μm ) and a mobile phase of Acetonitrile-Water (6：94).Flow rate was 1.0 mL/min,column temperature was 25 ℃.The detection wavelength was 260nm and sample loop volume 10 μl.Results:The calibration curve linear within the range of 1.25~20.00 μg /mL of acetonitrile,and the average recovery was 101.04% (RSD=1.93%).Conclusion:The method is simple,accurate and repeatable,which can be used for the quality control of Jianshen Pill. |
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