| 引用本文:孔思远1,2，吴蓉1,2，蔡双璠1,2，谭红胜1,2.夏枯草抗疱疹病毒多糖的提取及纯化工艺研究[J].世界中医药,2016,(07):. |
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| 夏枯草抗疱疹病毒多糖的提取及纯化工艺研究 |
| Extraction and Purification of Anti-HSV Polysaccharides from Prunella Vulgaris |
| 投稿时间：2016-07-05 |
| DOI：10.3969/j.issn.1673-7202.2016.07.002 |
| 中文关键词: 夏枯草 抗疱疹病毒 多糖 提取 纯化 醇沉 超滤 |
| English Keywords:Prunella Vulgaris L. Anti-HSV Polysaccharides Extraction Purification Alcohol precipitation Ultrafiltration |
| 基金项目:“重大新药创制”科技重大专项“十二五”计划（编号：2013ZX09103002-020） |
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| 中文摘要: |
| 目的：优化夏枯草抗疱疹病毒多糖的提取及纯化工艺，为新药研究提供科学依据。方法：以多糖含量和浸膏得率为指标，采用L9(34)正交试验法对夏枯草多糖提取过程中的提取次数，加水倍量及提取时间3个因素进行优化研究；以多糖含量及体外抗单纯性疱疹病毒I型（HSV-1）活性为考察指标，对超滤和醇沉两种纯化技术进行比较。结果：最优水提工艺为第1次加水14倍，第2、3次加水12倍，提取3次，每次提取时间1.5 h；采用超滤和醇沉两种技术分别纯化所得多糖。通过MTT法测得醇沉多糖和超滤多糖抑制HSV-1的IC50分别为（93.99±13.12）μg/mL和（51.35±15.30）μg/mL，结果表明超滤技术制备的多糖提取物抗单纯性疱疹病毒活性显著高于醇沉技术。结论：采用超滤技术制备的夏枯草多糖具有较高的含量及抗单纯性疱疹病毒活性，可作为夏枯草多糖开发新药的纯化工艺。 |
| English Summary: |
| The aim of this study is to optimize the extraction and purification process for anti-HSV polysaccharides from Prunella Vulgaris.Methods:The extraction method was modified by an orthogonal array experiment on three variable parameters：extraction time，solvent volume and extraction times.The content of polysaccharides and extraction yield were used as index.The effects of ultrafiltration technology and alcohol precipitation were compared in the purity of polysaccharide and anti-HSV activity.Results:The optimized extraction parameters are three extractions，1.5 hour extraction time，14 times of water for the first extraction and 12 times of water for the second and third extraction.The polysaccharide obtained from ultrafiltration technology and alcohol precipitation showed anti-HSV-1 activity in MTT assay，with IC50 values of(93.99 ± 13.12）μg/mL and(51.35 ± 15.30）μg/mL，respectively.The results suggested that the two purification processes have significantly different degrees of polysaccharide purity.The anti-HSV activity of polysaccharide from ultrafiltration technology was significantly higher than that of alcohol precipitation.Conclusion:The polysaccharides from Prunella Vulgaris obtained using ultrafiltration technology have higher anti-HSV activity than that isolated by alcohol precipitation. |
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