| 引用本文:于庆生1,2，经文善1，宋加奎1，张琦1,2，刘举达1,2.丹参及其联合5-FU对人胃癌SGC-7901细胞凋亡的影响[J].世界中医药,2016,(07):. |
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| 丹参及其联合5-FU对人胃癌SGC-7901细胞凋亡的影响 |
| The Effect of Salvia Miltiorrhiza and its Combination with 5-FU on Apoptosis of Human Gastric Cancer SGC-7901 Cells |
| 投稿时间：2015-08-23 |
| DOI：10.3969/j.issn.1673-7202.2016.07.043 |
| 中文关键词: 丹参 人胃腺癌SGC-7901细胞 凋亡 |
| English Keywords:Salvia Human gastric cancer SGC-7901 cells Apoptosis |
| 基金项目:十二五国家临床重点专科建设项目（编号：财社〔2013〕239号） |
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| 中文摘要: |
| 目的：探讨中药丹参对人胃癌SGC-7901细胞凋亡的影响。方法：采用Annexin V-FITC/PI双染法，使用流式细胞仪检测不同浓度丹参、5-FU及二者联合对人胃癌SGC-7901细胞的凋亡率影响。采用荧光显微镜观察不同浓度丹参、5-FU及二者联合对人胃癌SGC-7901细胞凋亡数量的影响。结果：1）不同浓度丹参溶液作用于人胃腺癌SGC-7901细胞后，细胞的凋亡率大于阴性对照组，且随药物浓度的增加而增加；2)丹参、5-FU联合用药作用于人胃癌SGC-7901细胞后，细胞的凋亡率大于单纯丹参组和5-FU组，且随丹参药物浓度的增加而增加；3）通过荧光显微镜观察可见联合用药组细胞数量少于阴性对照组，凋亡细胞数随药物浓度的增加而增多。结论：丹参能引起人胃癌SGC-7901细胞形态变化，能促进5-FU诱导胃癌SGC-7901细胞凋亡，且有剂量依赖性。 |
| English Summary: |
| To observe the effects of Salvia miltiorrhiza on apoptosis of human gastric cancer SGC-7901 cells. Methods: To test the effects of different concentrations of Salvia miltiorrhiza, different concentrations of 5-FU, and the mixtures on apoptosis rate of human gastric cancer SGC-7901 cells by flowing cytometry after AnnexinV-FITC/PI staining. To observe the effects of different concentrations of Salvia miltiorrhiza, different concentrations of 5-FU, and the mixtures on number of apoptosis of human gastric cancer SGC-7901 cells by fluorescence microscope. Results: 1) The apoptosis rate of human gastric cancer SGC-7901 cells that disposed with different concentrations of Salvia miltiorrhiza is higher than the control group, and the apoptosis rate has raised with the increase of concentration of Salvia miltiorrhiza.2) The apoptosis rate of human gastric cancer SGC-7901 cells that disposed with combination therapy group is higher than that of the group disposed by only Salvia miltiorrhiza or 5-FU, and the apoptosis rate increased with the increase of concentration of Salvia miltiorrhiza. 3)Using the Fluorescence microscopy, we can find that the number of the intact cells in Salvia miltiorrhiza combined with 5-Fu groups were all lower than the control group, and the number of apoptotic cells has increased with the drug concentration increasing. Conclusion: Salvia miltiorrhiza can cause human gastric cancer SGC-7901 cell morphological changes and accelerate 5-FU induce the apoptosis of cell, which was correlated with dose. |
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