世界中医药

目的：探讨钩藤中钩藤碱、异钩藤碱的提取与含量测定在预后中的应用。方法：采取水煎液以及甲醇提取液的方式对钩藤中的钩藤碱及异钩藤碱进行提取，并使用高效液相色谱系统分别对其提取的含量进行测定。结果：在2.5~80 μg/mL的范围内钩藤碱的线性关系良好，此时的回归方程为Y=76.717 0X-0.072 7（r=0.999 8），RSD＜8.7%；在2.0~80 μg/mL的范围内异钩藤碱的线性关系良好，此时的回归方程为Y=87.472 9X-0.366 6（r=0.999 7），RSD＜7.3%。甲醇提取液的方式所提取钩藤碱和异钩藤碱的含量高于水煎液的方式，并且使用二十倍质量的甲醇量，24 h的冷侵时间以及60 min的超声时间最合适。结论：使用甲醇进行提取在用于钩藤中钩藤碱及异钩藤碱中作用优异，对其质量的控制性好，重复性高，操作方法简单，可提高预后，值得在临床上应用推广。

English Summary:

To explore the application of extraction and content determination in the prognosis of uncaria rhynchophylline and isorhynchophylline in Gambir Plant Nod. Methods: The uncaria rhynchophylline and isorhynchophylline extract was taken by water extract and methanol extract methods, and the content of the extract was determined by high performance liquid chromatography. Results: The good linear relationship of rhynchophylline was in the range of 2.5~80 μg/mL, the regression equation for the time Y=76.717 0X-0.072 7(r=0.999 8),RSD＜8.7%; In the linear relationship between the range of 2.0~80 μg/mL, rhynchophylline was good, the regression equation for the time Y=87.472 9X-0.366 6(r=0.999 7), RSD＜7.3%. The content of rhynchophylline and isorhynchophylline extracted by methanol extraction liquid was higher than those extracted by the water. And the best way to extract the contents was under the condition of 20 times the maximum amount of methanol, 24 hours of the invasion of cold time and 60 min of sonication. Conclusion: The methanol extraction method is prominent in extracting in uncaria rhynchophylline and isorhynchophylline, with good quality control, high repeatability, simple operation, and effect of improving prognosis, which is worthy of clinical application and promotion.

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