世界中医药

目的：对忍冬藤中绿原酸的提取工艺进行研究。方法：采用正交设计法设计忍冬藤中绿原酸的微波提取工艺和超声波提取工艺，采用高效液相法测定各提取工艺下的绿原酸含量，并将两种提取方法得到的结果进行比较。结果：微波提取法的最佳提取工艺为：微波时间为60 s，乙醇浓度为50%，料液比为1：15，绿原酸含量为0.357%。超声波法最佳提取工艺为：提取功率为50 Hz，甲醇浓度为50%，料液比为1：20，绿原酸含量为0.321%。以绿原酸含量为指标，微波提取法优于超声波提取法。结论：忍冬藤中绿原酸的最佳提取方法为微波提取法，此方法时间短，耗能低，提取率优于超声波提取法，可用于忍冬藤中绿原酸的提取。

English Summary:

To study the extraction methods for chlorogenic acid in Lonicerae japonicae Caulis. Methods: The microwave extraction and ultrasonic extraction of chlorogenic acid in Lonicerae japonicae Caulis were set based on orthogonal design. The chlorogenic acid contents of different extraction techniques were detected by HPLC. The results of the two extraction methods were compared. Results: The results show that the chlorogenic acid was highest, up to 0.357%, when microwave time was set at 60s with 50% ethanol concentration and solid-liquid ratio of 1：15 by microwave extraction. The chlorogenic acid was highest when methanol concentration, up to 0.321%, was set at 50%, ultrasonic power 50W and solid-liquid ratio 1:20 by ultrasonic extraction. By taking chlorogenic acid content as an indicator, microwave extraction method was better than ultrasonic extraction. Conclusion: According to the results, the best extraction method for Chlorogenic acid in Lonicerae japonicae Caulis was microwave extraction with less time cost and low energy consumption and the extraction rate is better than that of the ultrasonic extraction. It can be applied in the extraction of chlorogenic acid in Lonicerae japonicae Caulis.

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