To observe the intervention effects of moxibustion and moxa smoke on oxidative stress indexes in ApoE-/-mice. Methods: Thirty-eight ApoE-/-mice (8 weeks old) were randomly divided into model group (n=6), sham-moxibustion group (n=8), cigarette smoke group (n=8), moxa smoke group(n=9), and moxibustion group (n=9). Thirteen same age C57BL/6 mice were used as blank controls. Mice in the blank control and model control groups were put into a glass box without any treatment. Mice of the sham and real moxibustion groups received moxibustion on Guanyuan (RN4) while the two smoke groups were exposed to smoke, whose concentration is 10 mg/m3. All the manipulations were performed 20 min/day, 6 days each week, 12 weeks totally. Levels of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) were determined by colorimetric method. Results: Comparing wit the model group, mice in the moxibustion group and moxa smoke group had significant lower level of MDA (P<0.05), and significant higher level of SOD (P<0.05). There was a remarkable decrease of MDA level (P<0.05) and an increase of SOD activity (P<0.05) in the moxibustion group compared to the sham-moxibustion group. MDA level was decreased (P<0.05) and SOD level was significantly increased (P<0.05) in moxa smoke group compared to those of the cigarette smoke group. Conclusions: Both moxibustion and moxa smoke can regulate the levels of serum MDA and SOD, which might be one of the mechanisms of their anti-oxidation effects for relieving the damage of vascular intima and atherosclerosis.