世界中医药

作者	单位
王昊，杨佳，赵百孝，哈略，刘耀萌，黄畅，于梦芸，惠鑫，和蕊，韩丽	北京中医药大学针灸推拿学院，北京，100029

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中文摘要:

目的：观察艾燃烧生成物即艾烟对载脂蛋白E基因敲除小鼠（ApoE-/-）大脑内神经递质5羟色胺（5-HT）和γ-氨基丁酸（GABA）含量变化的影响。方法：本实验选用8周龄载脂蛋白E基因敲除小鼠（ApoE-/-小鼠)21只，7只同龄相同遗传背景非转基因小鼠C57BL/6作为空白对照组。ApoE-/-小鼠随机平均分为艾烟组、模型对照组、氯吡格雷组。艾烟组小鼠5~15 mg/m3浓度艾烟干预，空白对照组和模型对照组小鼠空白抓取，氯吡格雷组口服给予氯吡格雷溶液14 mg/kg（1次/d），各组干预6 d/周，16周后牺牲动物，使用LC-MS/MS同时测定脑组织中神经递质GABA、5-HT含量。结果：艾烟组其脑内5-HT、GABA的含量显著高于模型组（P<0.05），氯吡格雷组脑内5-HT与模型组无明显差异，GABA的含量显著高于模型组（P<0.05），3组间均具有统计学意义（P<0.05）。结论：适当施灸时，一定浓度范围之内的艾燃烧生成物可以治疗性提高ApoE-/-小鼠脑内5-HT及GABA的含量。

English Summary:

To observe the effect of moxa smoke (Artemisia vulgaris) on neurotransmitters 5-HT and GABA in the brain of apolipoprotein E-deficient mice (ApoEKO) and explore the antiaging effect of moxa smoke. Methods: Seven eight-week old C57BL/6 mice were collected as control group, and twenty-one eight-week old ApoEKO mice were randomly divided into 3 groups (n=7/group): model group, moxa smoke group, and clopidogrel group. Mice in the smoke group was exposed to smoke, whose concentration is controlled within 5~15 mg/m3; mice in model group and control group were exposed to normal air. Mice in clopidogrel group were treated with 14 mg/kg clopidogrel orally once/day. After 16 weeks interference, the levels of 5-HT and GABA in the mice brain were measured. Results: Compared with the model group, the brain 5-HT and GABA levels of moxa smoke group were decreased significantly (P<0.05). There is no significant difference between the clopidogrel group and control group in 5-HT concentration, while GABA level was higher in clopidogrel group with significant difference (P<0.05), besides, the differences among the three groups have significant difference (P<0.05). Conclusion: Within appropriate concentration, moxa smoke can obviously increase brain 5-HT and GABA levels in ApoEKO mice.

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