To observe the effects of acupuncturing at “Qimen” (LR14)、“Tianshu” (ST25), “Yanglingquan” (GB34) on pressure of gallbladder, bile flow and motor function of oddi sphincter on guinea pig, so as to explore the efficacy and mechanism of acupuncture on regulating extra-hepatic biliary system movement and its advantages compared with the other different acpoints. Methods: Total 15 adult guinea pigs, no matter male or female, were were divided into LR14 group, ST25 group and GB34 group. The technology of implanted electrodes with double hooks was adopted for electrical detection of oddi sphincter, changes of gallbladder pressure, as well as bile flow. The frequency of acupuncture is 60r/min, acpoints were ST25、LR 14、GB34 for the single side, 1 min every time. And the results were obtained from the continues five time′s statistic. Results: Stimulation at ST25 or LR14 significantly increased the frequency and amplitude of oddi sphincter(P<0.05); but there was no significant decrease in the gallbladder pressure(P>0.05); On the contrary, stimulation at GB34 significantly decreased oddi sphincter and increased the gallbladder pressure(P<0.01). Bile current capacity of gallbladder had no significant change following one minute′s stimulation at the ST25 or LR14, but had significant change following one minute′s stimulation at the GB34(P<0.01). Conclusion: 1)Acupuncture at ST25 and LR14 could obviously inhibit the movement of gallbladder and promote the myoelectricity release of oddi sphincter. Acupuncturing at GB34 can apparently promote the shrinking movement of gallbladder and inhibit the myoelectricity release of oddi sphincter. 2)The effects of acupuncture at GB34 in this study had most significant changes than the ST25 or LR14.