| 引用本文:赵敬军1，荣培晶1，罗曼1，张金铃1，李亮1，梁繁荣2.针刺“阳陵泉”穴对豚鼠oddi括约肌肌电、胆囊压力及胆汁流量的影响[J].世界中医药,2016,(08):. |
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| 针刺“阳陵泉”穴对豚鼠oddi括约肌肌电、胆囊压力及胆汁流量的影响 |
| Effects on Guinea Pig′s Oddi Sphincter, Pressure of Gallbladder, Bile flow When Acupuncturing at GB34 |
| 投稿时间：2016-06-16 |
| DOI：10.3969/j.issn.1673-7202.2016.08.049 |
| 中文关键词: 胆囊压力 针刺 肌电 运动功能 oddi括约肌 豚鼠 |
| English Keywords:Gall bladder pressure Acupuncture Myoelectricity Motor function Oddi sphincter Guinea pig |
| 基金项目:科技部“973”课题（编号：2012CB518503）——“经穴效应循经特异性靶器官响应的生物学基础研究”，负责人：荣培晶 |
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| 中文摘要: |
| 目的：通过观察针刺“天枢”“期门”“阳陵泉”对豚鼠胆囊压力、oddi括约肌肌电及胆汁流量的影响，探讨针刺“阳陵泉”穴调节肝外胆道系统运动功能的效应是否优于其他穴位及其可能的作用机制。方法：选用成年豚鼠15只，雌雄不限，分成“天枢”“期门”“阳陵泉”组，实验采用oddi括约肌双钩金属电极埋植测肌电场电位与胆囊内置小型人工球囊技术测压，对麻醉豚鼠行在体记录oddi括约肌肌电活动及胆囊内压力变化，并记录干预前后胆汁流量情况，针刺激频率为60r/min，刺激穴位为：单侧“期门”“天枢”“阳陵泉”，每次刺激1 min，选取连续刺激5次的数据用来分析。结果：针刺“天枢”“期门”穴，oddi括约肌肌电频率与幅值与针刺前相比均明显增多(均P<0.05)，胆囊压力幅值无明显变化（P>0.05）；针刺“阳陵泉”，oddi括约肌肌电频率和幅值与针刺前相比明显下降（P<0.01），胆囊压力幅值明显上升（P<0.01）；针刺“阳陵泉”1 min，胆汁流量有显著性升高(P<0.001)，针刺“天枢”或“期门”1 min，胆汁流量略有升高但是无统计学意义（P>0.05）。结论：1）针刺豚鼠“期门”“天枢”能明显抑制胆囊的运动、促进oddi括约肌的肌电发放，减少进入十二指肠的胆汁流量，而针刺“阳陵泉”能显著促进胆囊的收缩运动、抑制oddi括约肌的肌电发放，促进豚鼠胆汁流量的增加;2）“阳陵泉”的效果要明显于其他穴位，由于“阳陵泉”为本经穴位，因此在本研究中存在明显的循经特异性。 |
| English Summary: |
| To observe the effects of acupuncturing at “Qimen” (LR14)、“Tianshu” (ST25), “Yanglingquan” (GB34) on pressure of gallbladder, bile flow and motor function of oddi sphincter on guinea pig, so as to explore the efficacy and mechanism of acupuncture on regulating extra-hepatic biliary system movement and its advantages compared with the other different acpoints. Methods: Total 15 adult guinea pigs, no matter male or female, were were divided into LR14 group, ST25 group and GB34 group. The technology of implanted electrodes with double hooks was adopted for electrical detection of oddi sphincter, changes of gallbladder pressure, as well as bile flow. The frequency of acupuncture is 60r/min, acpoints were ST25、LR 14、GB34 for the single side, 1 min every time. And the results were obtained from the continues five time′s statistic. Results: Stimulation at ST25 or LR14 significantly increased the frequency and amplitude of oddi sphincter(P<0.05); but there was no significant decrease in the gallbladder pressure(P>0.05); On the contrary, stimulation at GB34 significantly decreased oddi sphincter and increased the gallbladder pressure(P<0.01). Bile current capacity of gallbladder had no significant change following one minute′s stimulation at the ST25 or LR14, but had significant change following one minute′s stimulation at the GB34(P<0.01). Conclusion: 1)Acupuncture at ST25 and LR14 could obviously inhibit the movement of gallbladder and promote the myoelectricity release of oddi sphincter. Acupuncturing at GB34 can apparently promote the shrinking movement of gallbladder and inhibit the myoelectricity release of oddi sphincter. 2)The effects of acupuncture at GB34 in this study had most significant changes than the ST25 or LR14. |
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