Abstract:To explore the blood nourishing effect and mechanism of Albiflorin and Paeoniflorin on mouse model of blood deficiency syndrome induced by irradiation and its effect on levels of IL-3, GM-CSF, IL-6 and TNF-α. Methods: Mouse model of blood deficiency syndrome was induced by irradiation. Albiflorin and Paeoniflorin were given through gavage. The amount of WBC, RBC, HGB, index of thymus gland and spleen and the levels of GM-CSF, IL-3, IL-6 and TNF-α in serum were detected by radioimmunoassay. Results: Compared with the model group, the amount of WBC and index of thymus gland in the group of 30 mg/kg Albiflorin and 30 mg/kg Paeoniflorin were both increased significantly (P<0.01), and RBC increased in 15 mg/kg Albiflorin and 15 mg/kg Paeoniflorin significantly (P<0.05). The levels of IL-3 in serum in groups of 15 mg/kg Albiflorin, 30 mg/kg Paeoniflorin increased significantly (P<0.05). The levels of GM-CSF in serum in groups of 15 mg/kg Albiflorin, 30 mg/kg Paeoniflorin increased significantly (P<0.05), and the levels of GM-CSF in serum in group of 30 mg/kg Albiflorin increased significantly (P<0.01). The level of TNF-α in serum in groups of 30 mg/kg Paeoniflorin reduced significantly (P<0.05), and the level of TNF-α in serum in group of 30 mg/kg Albiflorin were reduced significantly (P<.001). Conclusion: Albiflorin, as a special compound in Debark Peony Root, which has the same hematopoietic effect as Paeoniflorin. Albiflorin and Paeoniflorin both can nourish blood and yin by cytokines regulation, which is the material basic of Debark Peony Root.