| 引用本文:王成龙，王林元，王景霞，朱映黎，赵丹萍，张建军.芍药内酯苷、芍药苷对放射线辐照致血虚小鼠IL-3,GM-CSF,IL-6及TNF-α的影响[J].世界中医药,2016,(08):. |
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| 芍药内酯苷、芍药苷对放射线辐照致血虚小鼠IL-3,GM-CSF,IL-6及TNF-α的影响 |
| Study of Blood Nourishing Effects of Albiflorin and Paeoniflorin on Blood Deficiency Mouse Model Induced by Irradiation of and Effect on Levels of IL-3, GM-CSF, IL-6 and TNF-α |
| 投稿时间：2016-03-31 |
| DOI：10.3969/j.issn.1673-7202.2016.08.050 |
| 中文关键词: 芍药内酯苷 芍药苷 放射线辐照法 血虚证 造血细胞因子 |
| English Keywords:Albiflorin Paeoniflorin Irradiation Blood deficiency syndrome Cytokines |
| 基金项目:国家自然科学基金项目(编号:81473370) |
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| 中文摘要: |
| 目的:研究芍药内酯苷、芍药苷对放射线辐照致血虚小鼠的补血作用及对白细胞介素-3(IL-3)、粒细胞-巨噬细胞集落刺激因子(GM-CSF)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)等造血细胞因子的调控机制。方法:采用放射线辐照法复制小鼠血虚模型,灌胃芍药内酯苷、芍药苷,检测小鼠外周血象、胸腺与脾脏指数及分离血清、血浆,分析IL-3、GM-CSF、IL-6、TNF-α的含量。结果:与模型组比较,芍药内酯苷30 mg/kg组及15 mg/kg组和芍药苷30 mg/kg组及15 mg/kg组均能升高白细胞值(P<0.01或P<0.05);芍药内酯苷30 mg/kg组及15 mg/kg组和芍药苷30 mg/kg组对红细胞值有升高作用(P<0.05);芍药内酯苷30 mg/kg组、芍药苷30 mg/kg组均能明显升高胸腺指数(P<0.01);芍药内酯苷30 mg/kg组、芍药苷30 mg/kg组明显升高IL-3含量水平(P<0.01);芍药内酯苷30 mg/kg组及15 mg/kg组和芍药苷30 mg/kg组均能升高GM-CSF含量水平(P<0.01或P<0.05);芍药内酯苷30 mg/kg组明显降低TNF-α含量水平,差异有统计学意义(P<0.01),芍药苷30 mg/kg组亦能降低TNF-α含量水平(P<0.05)。结论:芍药内酯苷作为白芍的特征性有效成分,与芍药苷一致具有补血作用;二者都通过对机体细胞造血因子的调控作用,促进血细胞的生成,维持造血功能的稳定,是白芍养血敛阴功效的物质基础。 |
| English Summary: |
| To explore the blood nourishing effect and mechanism of Albiflorin and Paeoniflorin on mouse model of blood deficiency syndrome induced by irradiation and its effect on levels of IL-3, GM-CSF, IL-6 and TNF-α. Methods: Mouse model of blood deficiency syndrome was induced by irradiation. Albiflorin and Paeoniflorin were given through gavage. The amount of WBC, RBC, HGB, index of thymus gland and spleen and the levels of GM-CSF, IL-3, IL-6 and TNF-α in serum were detected by radioimmunoassay. Results: Compared with the model group, the amount of WBC and index of thymus gland in the group of 30 mg/kg Albiflorin and 30 mg/kg Paeoniflorin were both increased significantly (P<0.01), and RBC increased in 15 mg/kg Albiflorin and 15 mg/kg Paeoniflorin significantly (P<0.05). The levels of IL-3 in serum in groups of 15 mg/kg Albiflorin, 30 mg/kg Paeoniflorin increased significantly (P<0.05). The levels of GM-CSF in serum in groups of 15 mg/kg Albiflorin, 30 mg/kg Paeoniflorin increased significantly (P<0.05), and the levels of GM-CSF in serum in group of 30 mg/kg Albiflorin increased significantly (P<0.01). The level of TNF-α in serum in groups of 30 mg/kg Paeoniflorin reduced significantly (P<0.05), and the level of TNF-α in serum in group of 30 mg/kg Albiflorin were reduced significantly (P<.001). Conclusion: Albiflorin, as a special compound in Debark Peony Root, which has the same hematopoietic effect as Paeoniflorin. Albiflorin and Paeoniflorin both can nourish blood and yin by cytokines regulation, which is the material basic of Debark Peony Root. |
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