| 引用本文:师一民1，王永强1，安辰1，赵晖1，齐放1，张秋霞1，陈振振1，樊永平2，李明1，王蕾1.补肾益髓方及其拆方对实验性自身免疫性脑脊髓炎小鼠脑和脊髓中p-Tau和Beta-tubulin表达的影响[J].世界中医药,2016,(09):. |
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| 补肾益髓方及其拆方对实验性自身免疫性脑脊髓炎小鼠脑和脊髓中p-Tau和Beta-tubulin表达的影响 |
| Effects of Bushen Yisui Decoction and Its Decomposed Decoctions on Expressions of p-Tau and Beta-tubulin in Brain and Spinal Cord of Mice with Experimental Autoimmune Encephalomyelitis |
| 投稿时间：2016-03-09 |
| DOI：10.3969/j.issn.1673-7202.2016.09.047 |
| 中文关键词: 补肾益髓方及其拆方 多发性硬化 实验性自身免疫性脑脊髓炎 P-Tau Beta-tubulin |
| English Keywords:Bushen Yisui Decoction and its decomposed decoctions Multiple sclerosis Experimental autoimmune encephalomyelitis p-Tau Beta-tubulin |
| 基金项目:国家自然科学基金项目（编号：81273742、81573898）；北京市教委重点项目（编号：KZ201310025023）；北京市属高等学校高层次人才引进与培养计划-长城学者项目（编号：CIT&TCD20140329） |
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| 中文摘要: |
| 目的：观察补肾益髓（Bu Shen Yi Sui，BSYS）方及其拆方补肾（Bu Shen，BS）和化痰活血（Hua Tan Huo Xue，HTHX）方对实验性自身免疫性脑脊髓炎（Experimental Autoimmune Encephalomyelitis，EAE）小鼠脑和脊髓中p-Tau和Beta-tubulin的作用。方法：C57BL/6小鼠雌性随机分为正常对照（NC）、模型（MO）、醋酸泼尼松（PA）、梓醇（CA）、BSYS、BS和HTHX组。造模当天与造模后第7天于小鼠背部两侧皮下注射抗原，即50 μg髓鞘少突胶质细胞糖蛋白（MOG）35-55、完全弗氏佐剂（CFA）与结核分支杆菌（TB）混合，并在免疫当天和2 d后腹腔注射500 ng的百日咳毒素（PTX）。每天对小鼠进行灌胃，观察其体重及神经功能评分，并于造模第20 d和第40 d分别取脑和脊髓进行病理检测。采用免疫组化法检测p-Tau和beta-tubulin的表达。结果：与MO组比较，BSYS、BS和HTHX组体重与神经功能评分均出现明显上升，HE染色可观察到炎细胞显著减少，核固缩减轻，同时，BSYS、BS和HTHX方均可明显下调p-Tau表达（P<0.05，P<0.01），上调beta-tubulin蛋白表达（P<0.05，P<0.01）。结论：BSYS方及其拆方BS和HTHX均对EAE小鼠有神经保护作用，表现在减少体重丢失，降低神经功能评分，减轻炎炎性细胞浸润，减轻轴突损伤及促进其修复，尤以BSYS全方更为显著。 |
| English Summary: |
| To observe effect of the Bushen Yisui Dection (BYD) and its decomposed Bushen Decoctions (BD) and Huatan Huoxue Decoction (HHD) on the expressions of p-Tau and beta-tubulin in the brain and spinal cord of mice with experimental autoimmune encephalomyelitis (EAE). Methods: C57BL/6 female mice were randomly divided into normal control (NC), model (MO) and prednisone acetate (PA), catalpol (CA), BYD, BD and HHD groups. The mice were injected subcutaneously with the antigens mixture, including 50 μg OG35-55, CFA and TB at two sites on the back on the 1st day and 7 days later, and then were injected intraperitoneally with 500 ng PTX on days 1st and 2 day later. The mice in each group were administered intragastrically once a day. Meanwhile, the body weight and neurological function of mice were observed. The brain and spinal cord of mice were removed for pathological examination on 20th and 40th day. The expressions of p-Tau and beta-tubulin were detected by immunohistochemistry analysis. Results: Compared with the MO group, the body weight and scores of mice were significantly increased in BYD, BD and HHD groups. HE staining showed a significant reduction in inflammatory cells and nuclear condensation. The expression of p-Tau was significantly reduced, while beta-tubulin was markedly increased in BYD, BD and HHD groups as well (P<0.05, P<0.01). Conclusion: BYD and its decomposed decoctions BD and HHD have neuroprotective effects on reducing weight loss and scores, inflammatory cell infiltration, axonal injury and enhancing the repair of axon in EAE mice. The effect of Bushen Yisui Decoction is more significant particularly. |
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