To observe effect of the Bushen Yisui Dection (BYD) and its decomposed Bushen Decoctions (BD) and Huatan Huoxue Decoction (HHD) on the expressions of p-Tau and beta-tubulin in the brain and spinal cord of mice with experimental autoimmune encephalomyelitis (EAE). Methods: C57BL/6 female mice were randomly divided into normal control (NC), model (MO) and prednisone acetate (PA), catalpol (CA), BYD, BD and HHD groups. The mice were injected subcutaneously with the antigens mixture, including 50 μg OG35-55, CFA and TB at two sites on the back on the 1st day and 7 days later, and then were injected intraperitoneally with 500 ng PTX on days 1st and 2 day later. The mice in each group were administered intragastrically once a day. Meanwhile, the body weight and neurological function of mice were observed. The brain and spinal cord of mice were removed for pathological examination on 20th and 40th day. The expressions of p-Tau and beta-tubulin were detected by immunohistochemistry analysis. Results: Compared with the MO group, the body weight and scores of mice were significantly increased in BYD, BD and HHD groups. HE staining showed a significant reduction in inflammatory cells and nuclear condensation. The expression of p-Tau was significantly reduced, while beta-tubulin was markedly increased in BYD, BD and HHD groups as well (P<0.05, P<0.01). Conclusion: BYD and its decomposed decoctions BD and HHD have neuroprotective effects on reducing weight loss and scores, inflammatory cell infiltration, axonal injury and enhancing the repair of axon in EAE mice. The effect of Bushen Yisui Decoction is more significant particularly.