| 引用本文:李木松，张贵贤，魏媛媛，刘震霞.茵陈蒿汤对肝纤维化大鼠肝组织内质网应激caspase-12通路的影响[J].世界中医药,2016,(09):. |
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| 茵陈蒿汤对肝纤维化大鼠肝组织内质网应激caspase-12通路的影响 |
| Effects of Yinchenhao Decoction on Endoplasmic Reticulum Stress Pathway of Caspase-12 in Hepatic Fibrosis Rats |
| 投稿时间：2015-09-21 |
| DOI：10.3969/j.issn.1673-7202.2016.09.050 |
| 中文关键词: 胆汁瘀积性肝纤维化 茵陈蒿汤 内质网应激 细胞凋亡 Caspase-12 |
| English Keywords:Cholestatic liver fibrosis Yinchenhao decoction Endoplasmic reticulum stress Apoptosis Caspase-12 |
| 基金项目: |
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| 中文摘要: |
| 目的:观察茵陈蒿汤对胆汁瘀积性肝纤维化大鼠肝组织内质网应激caspase-12通路的影响,探讨茵陈蒿汤抗胆汁瘀积性肝纤维的作用机制。方法:将35只SD大鼠分为假手术组和造模组,采用胆总管结扎法复制胆汁瘀积性肝纤维化大鼠模型,1周后将造模组动物分为模型组和中药组,中药组予以3.5 g/kg茵陈蒿汤灌胃,4周后处死动物,HE、Masson染色观察肝脏组织病理学变化;Western blot法检测各组大鼠肝脏GRP78、eIF2α和Caspase-12蛋白表达变化。结果:肝脏组织病理学变化显示模型组大鼠肝纤维化程度较假手术组明显增加(P<0.05),中药组大鼠肝纤维化程度较模型组减轻(P<0.05),但仍重于假手术组。Western blot结果显示,模型组肝脏GRP78、eIF2α和Caspase-12蛋白的表达与假手术组相比显著升高(P<0.01),中药组较模型组eIF2α和Caspase-12明显降低(P<0.01)。结论:抑制eIF2α和Caspase-12的活化,从而减少肝细胞凋亡,进而抑制内质网应激在胆汁瘀积性肝纤维化中的促进作用,是茵陈蒿汤抗胆汁瘀积性肝纤维化的作用机制之一。 |
| English Summary: |
| To observe the effects of Yinchenhao decoction on endoplasmic reticulum stress caspase-12 pathway of cholestasis liver fibrosis rat and its action mechanism on anti bile siltation liver fiber.Methods:Thirty five SD rats were divided into sham operation group and the duplicate model group. The common bile duct ligation method was used to replicate cholestasis liver fibrosis in rats. After 1 week of building, the rats were divided into model group and TCM group. TCM group was given3.5 g/kg Yinchenhao decoction by intragastric administration. The rats were killed after four weeks. HE and Masson staining method were used to observe the changes of the liver tissue pathology, and Western blot method was used to detect GRP78, eIF2 alpha and Caspase-12 protein expression changes in liver tissue of each group.Results:The liver histopathology examination showed that the liver fibrosis rat model was duplicated successfully. The degree of liver fibrosis in the TCM group was lower than that of the model group (P<0.05), but it was more severe than that of the Sham operation group. Blot Western showed that the expression of Caspase-12, eIF2α and GRP78 in the model group was significantly higher than those of the Sham operation group (P<0.01). Besides, eIF2α and Caspase-12 significantly decreased in the TCM group (P<0.01).Conclusion:Inhibiting eIF2α and caspase-12 activation, reducing apoptosis of liver cells, thereby inhibiting the promoting effect of ERS in the liver fibrosis of bile stasis is one possible action mechanism of Yinchenhao decoction in removing bile stasis liver fibrosis. |
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