To observe the effect of shikonin on endometrial cancer Ishikawa cells expression of estrogen signal pathway.Methods:Ishikawa cells cultured in vitro，after 1，3，5 μM Shikonin and the corresponding concentrations of shikonin were added 10 nm estrogen，MTT method were used to detect Ishikawa cells growth inhibition rate after 24，48，72 hours of treatment； Western blot and RT PCR method were applied to detect estrogen signaling pathway in ER alpha，cyclin D1，c-myc protein expression and ER alpha gene expression.Results:As shikonin concentration increased and intervention time extended，Ishikawa cell inhibition rate increased significantly； After 24 hours of shikonin intervention on Ishikawa endometrial cancer cells，ER alpha，CyclinD1，c-myc protein expression decreased，and the difference between the groups had statistical significance(P<0.05).But ER mRNA expression had no statistical difference，compared with the control group(P>0.05).Conclusion:As the concentration increased and time extended，shikonin significantly inhibited the proliferation of Ishikawa cells； through inhibiting the expression of ER alpha，cyclinD1 and c-myc protein expression，it prevented the endometrial carcinoma Ishikawa cell proliferation.