| 引用本文:黄彩梅1,2，夏亦冬1，胡国华1.紫草素对子宫内膜癌Ishikawa细胞雌激素信号通路表达的影响[J].世界中医药,2016,(09):. |
|
| 紫草素对子宫内膜癌Ishikawa细胞雌激素信号通路表达的影响 |
| Effect of Shikonin on Endometrial Cancer Ishikawa Cells Expression of Estrogen Signal Pathway |
| 投稿时间：2015-11-12 |
| DOI：10.3969/j.issn.1673-7202.2016.09.051 |
| 中文关键词: 紫草素 子宫内膜癌 雌激素信号通路 Ishikawa细胞 |
| English Keywords:Shikonin Endometrial cancer Estrogen signaling pathway Ishikawa cell |
| 基金项目:上海市卫计委科研基金课题（编号：20134118）；上海市卫生局进一步加快中医药事业发展三年行动计划项目“海派朱氏妇科流派传承研究”（编号：ZY3-CCCX-1-1013）；上海市中医药事业发展三年行动计划项目“胡国华上海市名老中医学术经验研究工作室”（编号：ZYSNXD-CC-MZY050）；上海市长宁区科委基金项目（编号：CNKW2015Y14） |
|
| 摘要点击次数: 753 |
| 全文下载次数: 805 |
| 中文摘要: |
| 目的：观察紫草素对子宫内膜癌Ishikawa细胞雌激素信号通路表达的影响。方法：Ishikawa细胞体外培养，经1、3、5M紫草素及相应浓度紫草素中分别加入雌激素10 nM，处理细胞后，用MTT法分别检测治疗24 h、48 h、72 h后Ishikawa细胞生长抑制率；Western-blot及RT-PCR法检测雌激素信号通路ERα、CyclinD1、c-myc蛋白表达情况及ERα基因的表达情况。结果：显示随着紫草素作用浓度的增加及干预时间的延长，Ishikawa细胞抑制率明显上升；紫草素作用于子宫内膜癌Ishikawa细胞24 h后，ERα、CyclinD1、c-myc蛋白表达下降，与空白对照组比较差异有统计学意义(P<0.05)，但是ERαmRNA表达无统计学意义，与空白对照组比较(P>0.05)。结论：紫草素随着浓度的增加及作用时间的延长，能够显著抑制Ishikawa细胞的增殖；紫草素通过抑制雌激素信号通路中ERα、CyclinD1、c-myc蛋白表达，起到抑制子宫内膜癌Ishikawa细胞增殖的作用。 |
| English Summary: |
| To observe the effect of shikonin on endometrial cancer Ishikawa cells expression of estrogen signal pathway.Methods:Ishikawa cells cultured in vitro，after 1，3，5 μM Shikonin and the corresponding concentrations of shikonin were added 10 nm estrogen，MTT method were used to detect Ishikawa cells growth inhibition rate after 24，48，72 hours of treatment； Western blot and RT PCR method were applied to detect estrogen signaling pathway in ER alpha，cyclin D1，c-myc protein expression and ER alpha gene expression.Results:As shikonin concentration increased and intervention time extended，Ishikawa cell inhibition rate increased significantly； After 24 hours of shikonin intervention on Ishikawa endometrial cancer cells，ER alpha，CyclinD1，c-myc protein expression decreased，and the difference between the groups had statistical significance(P<0.05).But ER mRNA expression had no statistical difference，compared with the control group(P>0.05).Conclusion:As the concentration increased and time extended，shikonin significantly inhibited the proliferation of Ishikawa cells； through inhibiting the expression of ER alpha，cyclinD1 and c-myc protein expression，it prevented the endometrial carcinoma Ishikawa cell proliferation. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
