| 引用本文:张毅，穆倩倩，于娜，赵丹丹，左加成，安宏，马越，莫芳芳，高思华.降糖消渴颗粒对自发性2型糖尿病KKAy小鼠肝脏功能和氧化应激的影响[J].世界中医药,2016,(09):. |
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| 降糖消渴颗粒对自发性2型糖尿病KKAy小鼠肝脏功能和氧化应激的影响 |
| Effect of Jiangtang Xiaoke Granule on Hepatic Function and Oxidative Stress in Type 2 Diabetic KKAy Mice |
| 投稿时间：2015-10-11 |
| DOI：10.3969/j.issn.1673-7202.2016.09.053 |
| 中文关键词: 降糖消渴颗粒 2型糖尿病 肝脏功能 氧化应激 |
| English Keywords:Jiangtang Xiaoke granule Type 2 diabetes Hepatic funciton Oxidative stress |
| 基金项目:重大新药创制（编号：2012ZX09103201-005）；科学研究与研究生教育-科学研究与科研基地建设项目-治疗代谢综合征创新中药临床前研究（编号：1000062520025） |
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| 中文摘要: |
| 目的：探讨降糖消渴颗粒对高脂饮食诱导的2型糖尿病KKAy小鼠肝脏功能和氧化应激的影响。方法：30只KKAy小鼠予以高脂饲料喂养4周后，建立2型糖尿病（空腹血糖≥13.9 mmol/L）小鼠模型。将成模后的KKAy小鼠随机分为模型组、吡格列酮组、降糖消渴颗颗粒高、中、低剂量组（1.75，3.5，7 g/kg），6只C57BL/6J鼠为正常对照组。干预10周后，检测小鼠血清中ALT、AST、γ-GT、SOD、MDA含量；取肝脏组织匀浆，检测肝脏组织中MDA、SOD、GSH的含量。结果：降糖消渴颗粒低剂量（1.75 g/kg）可降低血清ALT、γ-GT、MDA水平并提高SOD活性（P<0.01），但对肝脏中氧化应激指标无明显改善作用；中、高剂量（3.5，7 g/kg）均可显著降低血清ALT、γ-GT，减少血清及肝脏MDA含量，提高SOD活性（P<0.01），肝脏GSH活性也有所增强（P<0.05）。高剂量还具有降低血清AST的作用（P<0.01）。结论：降糖消渴颗粒可以有效改善T2DM状态下肝脏的功能和氧化应激状态。 |
| English Summary: |
| To investigate the effect of Jiangtang Xiaoke granule (JTXKG) on hepatic function and oxidative stress in type 2 diabetic KKAy mice. Methods: A total of 30 KKAy mice were fed by high-fat-diet for 4 weeks, T2DM model were established by FBG≥11.1 mmol·L-1. The T2DM mice were then randomly divided into model group, pioglitazone group and JTXK granule groups in low (1.75 g/kg), medium (3.5 g/kg), and high (7 g/kg) doses. The normal group had 6 C57BL/6J in it. After 10-week treatment, the serum ALT, AST, γ-GT, MDA, SOD and hepatic MDA, SOD, GSH were measured. Results: Compared with model group, T2DM mice supplemented with 1.75 g/kg dose of JTXK granule has lower serum ALT, γ-GT, MDA (P<0.01) and higher SOD activity (P<0.05). However, it has little effect on hepatic oxidative stress index. T2DM mice fed with 3.5 and 7 g/kg dose of JTXK granule has lower serum ALT, γ-GT, serum and hepatic MDA and higher SOD, GSH activities significantly (P<0.01). Conclusion: JTXKG can improve hepatic function and oxidative stress in T2DM mice. |
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