世界中医药

目的：探讨芪参益气滴丸对糖尿病大鼠视网膜谷氨酰胺合成酶及谷氨酸转运体的表达水平的影响。方法：选择维通利华实验动物研究所提供的六周龄SPF级SD大鼠80只，体重180~220 g，雄性，根据随机数字表法分为实验组（例数=70）、正常组（例数=10），进行链脲佐菌素(Streptozotocin，STZ)，一次性注射腹腔对胰岛β细胞进行选择性破坏，诱发实验性糖尿病；应用时由PH 4.4、0.1 mmol/L无菌的柠檬酸钠缓冲液配制为1% STZ溶液，根据大鼠体重予以65 mg/kg左下腹腔一次性注射1% STZ，正常组注射相同体积的生理盐水，72 h后应用快速血糖仪检测血糖水平；血糖水平超过16.7 mmol/L者则为糖尿病大鼠模型；随机分为对照组（多贝斯对照组）（20只）、治疗组（芪参益气滴丸组）（30只）、糖尿病模型组（模型组）（20只）、正常组（例数=10），应用RT-PCR法检测4组大鼠视网膜GFAP mRNA表达水平；应用免疫组织化学染色法(Labelled Streptavidin Biotin Method，LSAB法)检测4组视网膜的谷氨酸转运体(Glutamate/aspartate Transporter，GLAST)表达水平；应用免疫组织化学染色法（LSAB法）检测视网膜上谷氨酰胺合成酶（Glutamine Synthetase，GS)的表达水平情况。结果：对照组、模型组、治疗组的大鼠视网膜GFAP阳性表达IOD值显著低于正常组，差异有统计学意义（P<0.05）；治疗组、对照组的大鼠视网膜神经胶质原纤维酸性蛋白(Glialfibrillaryacidprotein，GFAP)阳性表达IOD值显著高于模型组，差异有统计学意义（P<0.05）；治疗组的大鼠视网膜GFAP阳性表达IOD值显著高于对照组，差异有统计学意义（P<0.05）；对照组、模型组、治疗组的大鼠视网膜GS阳性表达IOD值显著低于正常组，差异有统计学意义（P<0.05）；治疗组、对照组的大鼠视网膜GS阳性表达IOD值显著高于模型组，差异有统计学意义（P<0.05）；治疗组的大鼠视网膜谷氨酰胺合成酶（Glutamine Synthetase，GS)阳性表达IOD值显著高于对照组，差异有统计学意义（P<0.05）。结论：芪参益气滴丸可促进视网膜GS和GLAST的表达，降低谷氨酸的高浓度兴奋性毒性功能，保护视网膜神经细胞。

English Summary:

To investigate the effect of Shenqi Yiqi dripping pills on the expression of glutamine synthetase and glutamate transporters in the retina of diabetic rats.Methods:SD male rats 80,weight 180~220 g were randomly divided into experimental group (n=70) and normal group (n=10).The rats were modeled by STZ injection to induce experimental diabetes.The 1% STZ sterile solution was prepared by sodium citrate buffer PH4.4,0.1 mmol/L,and it was given according to the body weight of rats (65 mg/kg) through left lower abdominal cavity.Common saline were injected of the same volume on the normal group,and blood glucose level was examined by fast blood glucose meter examination after 72 h; mice whose blood glucose level over 16.7 mmol/L were categorized as the model diabetes group,and were randomly divided into the control group (calciumdobesilate control group) (n=20),treatment group (Qishen Yiqi group) (n=30),and diabetic model group (model group) (20 rats).RT-PCR method was used to detect the expression level of mRNA GFAP in four groups of rats.The expression level of glutamate transporter (GLAST) was detected by immunohistochemical staining (LSAB method) and the expression level of glutamine synthetase (GS) was detected by LSAB in four groups.Results:The positive expression of GFAP (Glial fibrillary acid protein) IOD value of the control group,model group,and treatment group were higher than the normal group with statistical difference (P<0.05).The GFAP IOD value in the treatment group and control group were higher than that of the model group (P<0.05).The GFPA IOD value in the treatment group was higher than that of the control group (P<0.05).The positive expression of GS (Glutamine synthetase) IOD value of the control group,model group,and treatment group were higher than the normal group with statistical difference (P<0.05).The GS IOD value in the treatment group and control group were higher than that of the model group (P<0.05).The GS value in the treatment group was higher than that of the control group (P<0.05).Conclusion:The expression of and GS and GLAST can be promoted by Qishen Yiqi dripping pills,which can low the toxin of high concentration of glutamate,and thus protecting retinal nerve cells.

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