| 引用本文:吴金娜1，龚旭初1，陈海东1，杨万富1，刘胜2.桔梗不同配伍对乳腺癌高转移潜能细胞4T1增殖及侵袭的影响[J].世界中医药,2016,(09):. |
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| 桔梗不同配伍对乳腺癌高转移潜能细胞4T1增殖及侵袭的影响 |
| Effect of Platycodon in Different Combination with Active Ingredients of Chinese Herbs on Multiplication and Metastasis of 4T1 Cell Line |
| 投稿时间：2015-01-27 |
| DOI：10.3969/j.issn.1673-7202.2016.09.055 |
| 中文关键词: 桔梗 配伍 4T1细胞 肺转移 |
| English Keywords:Platycodon Combination 4T1 cell line Lung metastasis |
| 基金项目:国家自然科学基金项目（编号：81173269、81102598）；南通市卫生局青年（编号：WQ2014053） |
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| 中文摘要: |
| 目的：应用MTT比色法及Transwell试验检测桔梗配伍不同中药对乳腺癌高转移潜能细胞4T1增殖及侵袭能力的影响。方法：给予SD大鼠中药煎剂灌胃，制备含药血清。应用MTT比色法检测桔梗不同配伍含药血清对4T1细胞增殖的影响；应用Transwell实验检测桔梗不同配伍含药血清对4T1细胞侵袭能力的影响。结果：桔梗及桔梗配伍不同中药组含药血清干预24 h后，对4T1细胞的增殖均有一定的抑制作用。各组含药血清对乳腺癌高转移潜能细胞4T1的增殖抑制率分别为桔梗组30.75%、桔梗加麦冬组34.44%、桔梗加蛇床子组44.71%、桔梗加莪术组49.84%。Transwell实验结果显示，桔梗及桔梗配伍不同中药各组均能降低透过小室的4T1细胞数，对于乳腺癌高转移潜能细胞4T1的侵袭能力有显著的抑制作用，同模型组比较差异有统计学意义（P<0.01）。同桔梗单用组比较，桔梗配伍不同中药组的抑制作用较强，其中桔梗配伍麦冬组及桔梗配伍蛇床子组效果显著，差异有统计学意义（P<0.01），桔梗配伍莪术组在抑制作用方面虽然由于桔梗单用组，但差异无统计学意义（P>0.05）。结论：桔梗及桔梗配伍不同治则中药对乳腺癌高转移潜能细胞4T1的增殖及侵袭有不同程度的抑制作用。 |
| English Summary: |
| To study the effects of platycodon in different combination with active ingredients of Chinese herbs on multiplication and metastasis of 4T1 cell line by MTT assay and transwell method.Methods:Preparation of medicated serum through SD mouse which Stock diet was given with traditional Chinese medicine apozem by stomach tube.Effects of medicated serum on multiplication and metastasis of 4T1 cell line were detested by MTT assay and transwell method.Results:The medicated serum of platycodon in combination with different active ingredients of Chinese herbs may inhibit the proliferation of 4T1 cell line after 24 hours.The inhibition rate of platycodon was 30.75%，platycodon plus ophiopogon japonicas was 34.44%，platycodon plus cnidium monnieri group was 44.71%，and platycodon plus rhizoma curcumae was 49.84%.Platycodon and Platycodon in combination with different active ingredients of Chinese herbs all can reduce the number of 4T1 cell line of throug the chamber by transwell method，they can inhibite the metastasis of 4T1 cell line significantly(compared with control，P<0.01).The inhibition of Platycodon in combination with different active ingredients of Chinese herbs was stronger than that of the Platycodon，and the inhibition of platycodon plus ophiopogon japonicas and platycodon plus cnidium monnieri group showed statistically significant differences(compared with Platycodon，P<0.01).The inhibition of platycodon plus rhizoma curcumae was stronger than that of the platycodon，showing no statistically differences(P>0.05).Conclusion:Platycodon in combination with different active ingredients of Chinese herbs can inhibite the multiplication and metastasis of 4T1 cell line. |
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