To study the effects of platycodon in different combination with active ingredients of Chinese herbs on multiplication and metastasis of 4T1 cell line by MTT assay and transwell method.Methods:Preparation of medicated serum through SD mouse which Stock diet was given with traditional Chinese medicine apozem by stomach tube.Effects of medicated serum on multiplication and metastasis of 4T1 cell line were detested by MTT assay and transwell method.Results:The medicated serum of platycodon in combination with different active ingredients of Chinese herbs may inhibit the proliferation of 4T1 cell line after 24 hours.The inhibition rate of platycodon was 30.75%，platycodon plus ophiopogon japonicas was 34.44%，platycodon plus cnidium monnieri group was 44.71%，and platycodon plus rhizoma curcumae was 49.84%.Platycodon and Platycodon in combination with different active ingredients of Chinese herbs all can reduce the number of 4T1 cell line of throug the chamber by transwell method，they can inhibite the metastasis of 4T1 cell line significantly(compared with control，P<0.01).The inhibition of Platycodon in combination with different active ingredients of Chinese herbs was stronger than that of the Platycodon，and the inhibition of platycodon plus ophiopogon japonicas and platycodon plus cnidium monnieri group showed statistically significant differences(compared with Platycodon，P<0.01).The inhibition of platycodon plus rhizoma curcumae was stronger than that of the platycodon，showing no statistically differences(P>0.05).Conclusion:Platycodon in combination with different active ingredients of Chinese herbs can inhibite the multiplication and metastasis of 4T1 cell line.