To study the overall change of chemical composition in Sichuan Dwarf Lilyturf Tuber after being made by wine so as to establish a method for determination of the content of methy lophiopogonanone A in wine made Sichuan Radix.Methods:Fresh Sichuan Dwarf Lilyturf Tuber and wine made Sichuan Dwarf Lilyturf Tuber were take to made liquid extraction by water.After that,the extraction was analyzed by HPLC,Waters Symmetry C18 column(4.6 mm× 250 mm,5 μm); the acetonitrile-0.2%Phosphoric acid water was used as the mobile phase; the column temperature was 30 ℃; the detection wavelength was set at 296 nm; Flow rate:1.0 mL·min-1; injection volume:20 μL.Results:The standard curve was drafted with sample concentration as abscissa,and chromatographic peak area value of the vertical axis.The regression equation of methyl Radix flavanones A was calculated as Y=15828ρ+ 42675,r=0.9998,with the linear range of 4.25~42.5 μg·mL-1.RSD of Methyl Radix flavanones A peak area was 0.85%,which showed that the methods had good precision.Reproducibility experiment showed the RSD of Methy lophiopogonanone A content was 0.67%,which showed good reproducibility.The average recovery of methyl Methy lophiopogonanone A was 97.31% (RSD1.54%),with the range of 95%~105%.It met the requirement.Comparative analysis of the chemical composition showed that the content of Methy lophiopogonanone A and B in Sichuan Dwarf Lilyturf Tuber improved significantly after being made by wine.Chemical composition of the wine made Sichuan Dwarf Lilyturf Tuber undergone significant changes in the quantity and quality.Conclusion:Wine made Sichuan Dwarf Lilyturf Tuber showed significant changes in the chemical composition because its overall changes of chemical composition and increase in active ingredients,which provides a theoretical basis for quality evaluation of Sichuan Dwarf Lilyturf Tuber.