| 引用本文:房玉涛，刘桂芳，张云.新活络效灵丹对ApoE基因敲除早期动脉粥样硬化小鼠TGF-β的影响[J].世界中医药,2016,(10):. |
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| 新活络效灵丹对ApoE基因敲除早期动脉粥样硬化小鼠TGF-β的影响 |
| Effect of New Huoluo Xiaoling Dan on the TGF-β in ApoE Knock-out Mice with Early Atherosclerosis |
| 投稿时间：2016-08-22 |
| DOI：10.3969/j.issn.1673-7202.2016.10.044 |
| 中文关键词: 新活络效灵丹 ApoE基因敲除小鼠 动脉粥样硬化 转化生长因子-β |
| English Keywords:New Huoluo Xiaoling Dan ApoE Knock-out Mice Atherosclerosis TGF-β |
| 基金项目:中国博士后科学基金（编号：20080440498）；国家自然科学基金青年项目（编号：81302964/H2708） |
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| 中文摘要: |
| 目的：观察新活络效灵丹对ApoE基因敲除小鼠AS早期斑块的病理形态学改变及转化生长因子-β(TGF-β)表达的影响，探讨新活络效灵丹抗AS早期斑块的可能机制。方法：采用高脂饲料喂养ApoE基因敲除小鼠建立AS模型，将45只小鼠随机分为模型组、西药组、中药组，另将C57BL/6J小鼠10只设为空白对照组。在造模的同时即给予药物干预，空白对照组不予处理，模型组予以生理盐水0.3 mL/d，中药组给予新活络效灵丹9.67 g/(kg·d),西药组给予阿托伐他汀钙片3.33 mg/(kg·d)，均每日灌胃1次，连续给药13周。取主动脉根部，HE染色进行病理观察，采用RT-PCR技术检测各组TGF-βmRNA的表达水平。结果：模型组主动脉内形成明显的AS斑块，并出现脂质核心,泡沫细胞增多，大量的巨噬细胞的浸润；西药组管壁可见AS斑块，斑块面积明显小于模型组；中药组斑块面积显著减少，部分内膜较为光滑。模型组小鼠主动脉组织中TGF-βmRNA表达水平明显低于空白对照组(P<0.01)；西药组、中药组小鼠主动脉组织中TGF-βmRNA表达水平明显高于模型对照组(P<0.05或P<0.01)，而中药组小鼠主动脉组织中TGF-βmRNA表达水平高于西药组，但2组比较差异无统计学意义(P>0.05)。结论：新活络效灵丹在一定程度上可通过上调保护性因子TGF-β以抑制免疫炎性反应，发挥抗动脉粥样硬化的作用,可能是其防治AS早期斑块的形成机制之一。 |
| English Summary: |
| To observe the effect of New Huoluo Xiaoling Dan (NHLXLD)on aorta pathological changes in ApoE Knock-out mice and the expressions of Transforming Growth Factor beta (TGF-β),and to explore possible mechanism of anti-AS．Methods:AS model was induced by high lipid diet in ApoE knock-out mice.Forty five ApoE knock-out mice were equally randomized into 3 groups， including control group(CG),western medicine group (WMG) and traditional Chinese medicine group(TCMG).Ten C57BL /6J mice were also prepared as blank group (BG).During modeling,BG received no intervention;CG took normal saline 0.3 mL daily;TCMG was treated by NHLXLD 9.67 g/kg.d,and WMG was administered avarstatin 3.33 mg/kg.d.The treatment lasted for 13 weeks with gastric lavage once everyday.Then the aortic roots were taken for HE stain and the mＲNA levels of TGF-βof each group were assayed．Results:Atherosclerotic plaques were observed obviously in the aorta of CG mice with overwhelming formation of foam cells, lipid core and a large number of macrophages infiltration．There were visible AS plaques on WMG aortic wall;plaque area was significantly lower than the CG;plaque area in TCMG was significantly reduced with the more smooth aortic endometrium. The TGF-β mRNA expressions of CG were lower than BG(P<0.01);The TGF-β mRNA expressions of WMG and TCMG were higher than CG(P<0.05 or P<0.01).While the TGF-β mRNA expressions of TCMG were higher than WMG, but the difference was not statistically significant (P>0.05).Conclusion:NHLXLD suppressed immune inflammatory response in a certain extent by increasing protective factor TGF-β on anti-AS.It might be one of the possible mechanisms preventing the formation of AS plaque． |
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