To observe changes of the expression of neuropeptide Y (NPY) and protein gene product 9.5 (PGP9.5) in urethral tissue of rats with stress urinary incontinence (SUI) and the effect of tuina on abdomen or lumbago, providing research evidence for tuina therapy in treating SUI. Methods:Samples at urethra were obtained from the rat models of SUI, normal rats, tuina on abdomen rats and tuina on lumbago rats and the expression levels of NPY and PGP9.5 were detected by immunohistochemistry. Results:The rat models of SUI were established create birth injury and menopause. Immunohistochemistry showed that NPY and PGP9.5 existed in urethral tissue, and the staining intensities of NPY and PGP9.5 in rat model group decreased significantly compared with normal control group, tuina on abdomen group and tuina on lumbago group (P<0.05). There was no significant difference of the expression of NPY between tuina on abdomen group and tuina on lumbago group. Compared with tuina on lumbago group, the expression of PGP9.5 in tuina on abdomen group decreased significantly (P<0.05). Conclusion:The therapeutic effects of tuina on abdomen or lumbago for SUI may have certain connection with repairing the neurogenic injury. This therapy reflects the theory of “inducing Yang from Yin and inducing Yin from Yang”.