| 引用本文:李小燕1，杜成芬1，肖敏1，夏俊琳1，赵娟1，何华琼2.丹参酮ⅡA磺酸钠注射液对大鼠急性心肌缺血预处理VEGF的影响[J].世界中医药,2016,(10):. |
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| 丹参酮ⅡA磺酸钠注射液对大鼠急性心肌缺血预处理VEGF的影响 |
| Effect of Sodium SulfonateⅡ A Sulfonate Injection on VEGF of Rats with Acute Myocardial Ischemia Preconditioning |
| 投稿时间：2015-11-09 |
| DOI：10.3969/j.issn.1673-7202.2016.10.046 |
| 中文关键词: 丹参酮ⅡA磺酸钠 急性心肌缺血预处理 血管内皮生长因子 |
| English Keywords:Sodium Sulfonate A Sulfonate Acute myocardial ischemia preconditioning Vascular endothelial growth factor |
| 基金项目:十堰市科技局项目（编号：ZD2013013） |
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| 中文摘要: |
| 目的：观察丹参酮ⅡA磺酸钠注射液对大鼠急性心肌缺血/再灌注损伤缺血预处理后血管内皮生长因子（Vascular Endothelial Growth Factor，VEGF）表达的影响。方法：16只健康大鼠随机分为缺血/再灌注组、丹参酮预处理组，丹参酮预处理组经尾静脉注射1%丹参酮ⅡA磺酸钠注射液，缺血/再灌注组给予等体积生理盐水预处理。于预处理14 d后采用结扎左侧冠状动脉前降支30 min再复灌30 min的方法建立大鼠急性心肌缺血/再灌注损伤模型。检测缺血预处理前后静脉血超氧化物歧化酶(Superoxide Dismutase，SOD)、过氧化氢酶(Catalase，CAT)活性及缺血心肌组织丙二醛（Malondialdehyde，MDA），心肌缺血区组织VEGF及VEGF-mRNA、缺氧诱导因子-1a(Hypoxia Inducible Factor-1a，HIF-1a)及HIF-1a mRNA、受体胎肝激酶-1(Fetal Liver Kinase 1，FLK-1)及FLK-1mRNA表达。结果：与缺血/再灌注组比较，丹参酮预处理组VEGF及VEGF-mRNA、FLK-1、FLK-1mRNA、HIF-1a、HIF-1amRNA的表达均明显增强，SOD及CAT活性明显提高，MDA降低（P<0.05）。结论：丹参酮ⅡA磺酸钠预处理可提高VEGF表达及超氧化物歧化酶活性，对急性心肌缺血/再灌注损伤大鼠心肌有一定的保护作用。 |
| English Summary: |
| To observe the effect of sodium sulfonate A sulfonate injection on the expression of vascular endothelial growth factor(VEGF)in rats with acute myocardial ischemia / reperfusion injury.Methods:Sixteen healthy rats were randomly divided into two groups，ischemia / reperfusion group and Salvia miltiorrhiza group.The two rats were treated with 1% sodium sulfonateⅡ A sulfonate injection and sodium chloride injection from caudal vein.After fourteen days，rats underwent a 30 min left anterior descending coronary artery occlusion and a 30 min reperfusion.This process was repeated 3 times.Mycardial tissue and serum were assessed by Western blot，quantitative RT-PCR and assay kit.Results:The expression of FLK-1，FLK-1mRNA，HIF-1a，HIF-1amRNA，VEGF and VEGF-mRNA were increased.The activity of SOD and CAT were significantly enhanced，however the content of MDA was decreased compared with IR.The difference showed statistically significant(P<0.05).Conclusion:Sodium Sulfonate A Sulfonate may enhance the expression of VEGF and the activity of SOD.It has certain protective effects on rats with acute myocardial ischemia / reperfusion injury. |
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