世界中医药

基金项目:国家自然科学基金项目（编号：81260667）；广西科技厅面上项目（编号：2011GXNSFA018204）；广西卫生厅中医药科技专项课题（编号：GZKZ10-050）；广西重点实验室建设项目（编号：15-140-31、14-045-12、13-051-06）；广西农作物废弃物功能成分研究协同创新中心建设（编号：CICAR2015-Z1）

作者	单位
曾春晖1，李先梅1，2，蔡妮娜1，3，谭娥玉1，4，覃文慧1，韦乃球1，秦树森1，5，马雯芳1，杨柯1	1 广西中医药大学药学院，南宁，530001； 2 广西钦州市卫生学校，钦州，535000； 3 南宁市中医医院，南宁，530001； 4 江门市五邑中医院，江门，529000； 5 桂林市妇女儿童医院，桂林，541001

摘要点击次数: 715

全文下载次数: 1131

中文摘要:

目的：摸索简单易行的方法诱导草酸钙型肾结石小鼠模型，为后续药效学研究提供可靠的动物模型。方法：将健康小鼠分为正常组（A组）、模型组（B组）、模型组（C组），B组灌胃给予诱石剂（含075%乙二醇和075%氯化铵的水溶液），C组灌胃给予诱石剂（含1%乙二醇和1%氯化铵的水溶液），1 mL/次，3次/d，连续28 d进行造模，A组平行灌胃给予等量生理盐水。实验期间观察小鼠的进食进水情况，生命体征，行为活动及体毛变化，于造模后7、14、21、28 d收集24 h尿液进行草酸和钙含量的测定，于末次造模后处死小鼠，剖取肾脏进行病理学观察。结果：于造模第3天开始，B、C组小鼠均出现后肢无力爬行，食欲减退，体毛呈进行性晦暗无光泽，C组小鼠的表现比B组小鼠严重；造模7 d后，模型组开始出现不同数量的动物死亡，且C组死亡率高于B组；B组和C组小鼠尿液中草酸、钙浓度均明显高于A组（P<001），B组尿草酸浓度明显高于C组（P<005~001），而尿钙浓度明显低于C组（P<001）；病理学结果表明，A组小鼠肾小管未见扩张和草酸钙晶体沉积，B组、C组小鼠肾组织均出现肿胀，肾小管管腔明显扩张、变性、坏死，肾间质有明显慢性炎性细胞浸润，表面点状分布白色结晶或钙化，在近曲小管和远曲小管有大片结晶存在，且B组的成石率比模型C组高。结论：采用075%乙二醇及075%的氯化铵混合溶液作为诱石液，可成功诱导死亡率低、成石率高的草酸钙型肾结石小鼠模型。

English Summary:

To explore a method of establishing mice model with calcium-oxalate kidney stones so as to provide the reliable animal models for follow-up study on pharmacodynamics. Methods: Mice were divided to normal group (A group), model group (B group) and model group (C group) randomly. 075% ethylene glycol and 075% ammonia chloride mixture were administrated orally1 mL/time and 3 times/day to B group mice for 28 consecutive days. 1% ethylene glycol and 1% ammonia chloride mixture were administrated orally to C group mice as well as B group. The same volume of normal saline was administered in parallel to A group. Food and water intake, vital signs, behavior and change of hair were observed in experiment. 24 h urine of mouse was collected for detecting concentration of oxalic acid and calcium at 7, 14, 21, 28 days after modeling. All of mice were sacrificed at the last time, and took the kidney to carry out pathological observation. Results: The phenomenon of inability to crawl on the hind legs, loss of appetite and the dull hair were observed in B and C group mice after modeling 3 days. The situation of C group was more serious than B group. The model animals began to die after modeling 7 days and the mortality rate of C group was higher than that of group B. The concentration of oxalic acid and calcium in urine of B group or C group were more than A group (P<001). The concentration of oxalic acid in B group was significantly higher than that in C group(P<005~001), but the calcium concentration was significantly lower than that of the C group(P<001). No abnormalities and Calcium oxalate crystal were found in the kidney of A group mice. In B and C groups, the renal tissues of mice were swollen. The renal tubular lumen showed obvious expansion, degeneration necrosis and there was obvious chronic inflammatory cell infiltration in renal interstitial. The surface of the kidney is dotted with white crystals or calcification. There are large tracts of crystal in the proximal and distal tubules and Number of crystals in B group was more than that in C group. Conclusion: It was found that 075% ethylene glycol and 075% ammonia chloride mixture can be successfully induced low mortality, high stone rate of calcium oxalate kidney stones in mice.

查看全文查看/发表评论下载PDF阅读器