To observe the protection effects of Fufang yizhi Granule on the cellular damage induced by the protein Aβ25-35,and to be the basis of further study on the mechanism of this product to improve the memory in Alzheimer’s disease. Methods: Different processing cells were divided into Normal control group, Aβ25-35model control group, Aβ25-35+Normal serum group, and Aβ25-35+CYZG serum group, then given different procedure. Normal control group: cultured with DMEM medium for 48 h, Aβ25-35model control group, 24 h after being cultured with DMEM medium, then added the medium contained 20 μMAβ25-35and continuously cultured 24 h. Aβ25-35+Normal serum group: 24 h after being cultured with DMEM medium contained normal serum, then add the medium contained 20 μMAβ25-35and continuously cultured 24 h, Aβ25-35+CYZG serum group: 24 h after being cultured with DMEM medium contained CYZG serum, then add the medium contained 20 μMAβ25-35and continuously cultured 24 h. Results: Protein Aβ25-35 could time-dependently inhibit the growth of PC12 cells, CYZG serum of different concentrations had protective effect on the cellular damage, with the increase of the CYZG serum dosage, the cell survival rate increase, and growth inhibition rate decrease. Protein Aβ25-35 could induce the apoptosis of PC12 cells, CYZG serum of different concentrations significantly inhibited the apoptosis. Conclusion: Fufang yizhi Granule could protect the PC12 from the damage induced by Protein Aβ25-35, the mechanism of neuroprotective effect of Fufang yizhi Granule may relate with the inhibition effect of apoptosis.