世界中医药

目的：研究大高良姜、高良姜、益智、草豆蔻、红豆蔻5味山姜属中药对胃溃疡寒证大鼠cAMP、cGMP含量的影响。方法：分空白组、模型组、附子理中丸阳性对照组、5味受试药物高低剂量组，采用15%冰乙酸加4 ℃知母水煎液造胃溃疡寒证模型。造模成功后给药，采用ELISA法测定血浆、血小板、血管平滑肌、胃组织cAMP、cGMP含量，计算cAMP/cGMP比值。结果：与模型组比较，除血小板益智低剂量组外，其余9个受试组血浆、血小板、血管平滑肌、胃组织cAMP含量均升高（P<001或P<005）；除血小板益智低剂量组，胃组织草豆蔻、红豆蔻、益智低剂量组外，其余受试组血浆、血小板、血管平滑肌、胃组织的cAMP/cGMP比值明显增高（P<001或P<005）。各受试组cGMP含量差异均无统计学意义。结论：5味山姜属中药能显著升高胃溃疡寒证大鼠血浆、血小板、血管平滑肌、胃组织cAMP含量及cAMP/cGMP比值，这可能是5味山姜属中药温热药性和温胃散寒作用的科学内涵。

English Summary:

To study the effect of the five Alpinia Rox(Alpinia officinarum Hance, Alpinia galangal(L.)willed, Alpinia katsumadai Hayata, Alpinia galangal, Alpinia oxyphyllaMiq) on cyclie Adenosine monnphosphate (cAMP) levels, cyclie guanosine monophosphate (cGMP) levels, and ratio of cAMP/cGMP in stomach excessive cold syndrome rats. Methods:Rats were divided into blank control group, model group, 2 positive control group, and 10 warm-heat herb groups, with 10 rats in each group. Rats model of stomach excess-cold syndrome was established by intragastric infusion of the 15% glacial acetic acid and 4 ℃ Common Anemarrhena Rhizome decoction liquid. After establishing the model, blank group and model group were given water, warm-heat herb respectively. The contents of cAMP, cGMP of stomach tissue, serum, vascular smooth muscle platelet were detected by the method of enzyme-linked immunoassay. Meanwhile, the cAMP/cGMP ratio was calculated. Results: Compared with model group, the cAMP level of 10 subjects content in rat stomach tissue, serum,thrombocyte and vascular smooth muscle were higher (P<001,P<005); no obvious difference in cGMP content; except for low doses in Alpinia katsumadai, Galanga Galangal Seed and Alpinia oxyphylla, ratio of cAMP/cGMP in rest drug participants increased obviously (P<001,P<005). Conclusion: Five Alpinia Roxb may elevate levels of cAMP, cAMP/cGMP ratio in model rats with stomach excessive cold syndrome, which may reveal the fact that five Alpinia Roxb have the effect of warming stomach for dispelling cold.

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