世界中医药

作者	单位
高占义1，魏月娟1，王彦敏2，李二娟1，张姗1，王庆海3	1 河北医科大学附属沧州中西医结合临床学院心内科，沧州，061000； 2 河北医科大学附属沧州中西医结合临床学院超声科，沧州，061000； 3 河北医科大学附属沧州中西医结合临床学院科教科，沧州，061000

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中文摘要:

目的：探讨自拟强心汤对心梗后心力衰竭大鼠心肌舒缩功能的作用机制。方法：采用结扎左冠状动脉法制备大鼠心梗后慢性心力衰竭动物模型；8周后，给予药物灌胃治疗；经治疗4周后，采用通过颈动脉插管测定大鼠血流动力学状态，通过实时荧光定量PCR（FQ-PCR）法和Western Bolt法检测心肌钙调控蛋白肌浆网钙泵（SERCA2a）、雷尼丁受体2（RYR2）mRNA水平及其蛋白表达量。结果：模型组大鼠的心功能显著下降，主要表现在血流动力学参数改变，其中左室舒张末期压（LVEDP）明显升高（P<001），左室收缩压（LVSP）、左室上升最大速率（+LVdp/dtmax）及左室下降最大速率（-LVdp/dtmax）绝对值均降低（P<005）；心肌钙调控蛋白SERCA2a、RYR2 mRNA水平及其蛋白表达量均明显下降（P<005）。给予自拟强心汤干预后，能够有效的降低心力衰竭大鼠LVEDP水平（P<005），升高LVSP、+LVdp/dtmax、-LVdp/dtmax水平（P<001）；能够升高心肌钙调控蛋白SERCA2a、RYR2 mRNA及其蛋白表达量的水平（P<005）。结论：自拟强心汤能够有效的改善心力衰竭大鼠的心功能，其机制可能与升高心肌钙调控蛋白SERCA2a、RYR2的蛋白表达量，改善钙循环有关。

English Summary:

To clarify myocardial systolic and diastolic function and possible mechanism of Qiangxin Tang on the model of heart failureMethods:Model of heart failure after myocardial infarction was prepared by left coronary artery ligationAfter 8 weeks，take a lavage treatment every day；After 4 weeks of continuous administration，cardiac hemodynamics parameters were recorded and SERCA2a，RYR2 mRNA level were detected by PCR（FQ-PCR）and SERCA2a，RYR2 protein expression were detected by Western boltResults:compared with control group，cardiac function of model group is lower：hemodynamic parameters LVEDP is increased obviously(P<001),LVSP，+LVdp/dtmax and-LVdp/dtmax absolute values were all lower(P<005)，and the calmodulin SERCA2a and RYR2 mRNA and protein relative expression of model group rats was decreased(P<005)Compared with model group，Qiangxin Tang group could reduce LVEDP levels(P<005),increase LVSP，+LVdp/dtmax and-LVdp/dtmax levels(P<001),and the calmodulin SERCA2a and RYR2 mRNA and protein relative expression of Qiangxin Tang were higherConclusion:Qiangxin Tang can effectively improves cardiac function in heart failure rats; Its mechanism is related with improving calmodulin SERCA2a、RYR2 and improving calcium cycle.

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