| 引用本文:罗云春，易文，姚宇宙，朱妮.溃结方对溃疡性结肠炎大鼠血清炎性反应因子与肠黏膜修复作用的研究[J].世界中医药,2016,(11):. |
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| 溃结方对溃疡性结肠炎大鼠血清炎性反应因子与肠黏膜修复作用的研究 |
| Effects of Kuijie Formula on Expression of Inflammatory Factors and Repair Intestinal Mucosa on Rat Blood Serum |
| 投稿时间：2016-03-15 |
| DOI：10.3969/j.issn.1673-7202.2016.11.045 |
| 中文关键词: 溃疡性结肠炎 溃结方 炎性反应 JAK2/STAT3信号通路 |
| English Keywords:Ulcerative colitis Kuijie Formula Inflammatory response JAK2/STAT3 signal pathway |
| 基金项目:湖北省中西医结合科研项目（编号：2013Z-Y16） |
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| 中文摘要: |
| 目的：观察溃结方对溃疡性结肠炎（UC）大鼠炎性反应因子及JAK2/STAT3信号通路的影响，并探讨其可能的作用机制。方法：采用2，4，6-三硝基苯磺酸（TNBS）/乙醇溶液灌肠法建立溃疡性结肠炎大鼠模型，每日灌胃给予溃结方治疗，酶联免疫吸附法（ELISA法）测定UC大鼠血清促炎因子IL-6、TNF-α及抗炎因子IL-10、IL-4的水平，肉眼观察大鼠结肠病理形态学改变并进行评分，免疫组织化学法（SP法）测定结肠组织中JAK2和STAT3蛋白的表达。结果：溃结方可显著降低UC大鼠血清IL-6、TNF-α水平，同时升高抗炎因子IL-10、IL-4，差异均有统计学意义（P<005）；溃结方可明显降低UC大鼠结肠DAI及CMDI评分（P<005），同时溃结方可减少结肠组织中JAK2和STAT3蛋白的表达。结论：溃结方可调节UC大鼠体内促炎因子和抗炎因子的平衡，减缓炎性反应，改善UC大鼠结肠溃疡损伤，可能的机制是与其减少JAK2和STAT3蛋白的表达相关。 |
| English Summary: |
| To observe the effects of Kuijie Formula on the expression of inflammatory factors and JAK2，STAT3 in ulcerative colitis rats and to explore its mechanism.Methods:TNBS solution was used to build the ulcerative colitis rats models and Kuijie Formula was administrated by gavage everyday.Enzyme-Linked Immuno Sorbent Assay(ELISA)method was used to test UC rat’s TNF-α，IL-6，IL-10，IL-4； the pathological changes of the colon in rats was observed by naked eyes.JAK2 and STAT3 were determined by immunohistochemical staining(SP method).Results:Kuijie Formula can reduce the TNF-α，IL-6 levels and level up the IL-10，IL-4 in serum significantly(P<005).It can also reduce the DAI and CMDI sores of the rats’ colon as well as the expression of JAK2 and STAT3 in colon tissues.Conclusion:Kuijie Formula can regulate the balance of pro-inflammatory and anti-inflammatory cytokines in UC rats，slow down the inflammatory response and improve the colonic ulcer damage in UC rats.Its mechanism may be related to reducing the inflammatory response and the expression of JAK2 and STAT3 |
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