| 引用本文:何旭云1，贺姣姣1，郑宁宁1，王顺春2，李后开1.黄芪多糖对肥胖小鼠的减肥作用与调节肠道菌群的关系研究[J].世界中医药,2016,(11):. |
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| 黄芪多糖对肥胖小鼠的减肥作用与调节肠道菌群的关系研究 |
| Study on Anti-Obesity Effect and Modulation of Gut Microbiota by Astragalus Polysaccharides in Mice |
| 投稿时间：2016-10-27 |
| DOI：10.3969/j.issn.1673-7202.2016.11.046 |
| 中文关键词: 黄芪多糖 肥胖 肠道菌群 胰岛素敏感性 脂肪变性 16S rDNA |
| English Keywords:Astragalus polysaccharides Obesity Gut microbiota Insulin sensitivity Hepatic steatosis 16S rDNA |
| 基金项目:国家自然科学基金项目（编号:81673662）,上海市科委“浦江人才”计划（编号:14PJD031），上海市教委一流学科创新项目(编号：ZYX-CXYJ-017)，上海市高校特聘教授（东方学者）人才计划（2014），上海市教委“曙光学者”人才计划（2016） |
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| 中文摘要: |
| 目的：观察黄芪多糖（Astragalus Polysaccharides，APS）对于高脂饮食诱导的肥胖小鼠的减肥作用与调节肠道菌群的关系。方法：1）将50只C57bl/6J小鼠随机分为5组（n=10），分别为正常对照组（Con）、HFD（high-fat diet）组和APS低中高（在高脂饮食中添加分别添加2%，4%，或8%的APS）剂量组。利用高脂饮食连续喂养8周诱导出小鼠肥胖模型，给药组同步喂养，每周进行称重。实验周期结束后，收集各组粪便样本，利用基于细菌16S rDNA测序的元基因组学方法，分析了APS对于高脂喂养小鼠肠道菌的影响。2）将10只C57bl/6J小鼠随机分为2组（n=5），分别为HFD_R组（HFD_Receptor）和APS_R组（APS_Receptor），每天分别灌胃来自HFD组和APS低剂量组（2%APS）小鼠的新鲜粪便提取液进行肠道菌移植，前八周给予正常饮食，后四周更换为HFD。结果：APS能够明显抑制高脂喂养小鼠肥胖的形成、减轻肝脏脂肪变性、降低肝脏TG水平、改善胰岛素敏感性、显著恢复高脂喂养小鼠的肠道菌群紊乱，增加拟杆菌（Bacteroidetes）与厚壁门（Firmicutes）菌的相对丰度、降低变形菌门（Proteobacteria）细菌的相对丰度，并且，APS的减肥效应能够通过肠道菌移植转移给高脂喂养受体小鼠。结论：APS对高脂喂养小鼠具有减肥作用，且APS的减肥作用与调节肥胖小鼠的肠道菌群有关。 |
| English Summary: |
| To explore relationships between the anti-obesity effect and modulation of gut microbioata by APS in high-fat diet (HFD)-fed mice.Methods:1 A total of 50 male C57bl/6J mice were randomly divided into five groups including control group (Con), HFD group, and three APS groups with low, middle and high dosages (2 %, 4 %, 8 % APS mixed in HFD). All animals were fed with corresponding diet for consecutive eight weeks during which their body weight was measured weekly. At the end of the experiment, fecal samples were collected for analysis of gut microbiota by 16S rDNA sequencing approach. 2 To investigate the contribution of gut microbiota to body weight reduction of APS, two groups of mice (n=5/each group) were transplanted with intestinal bacteria from mice that were fed with or without APS (2 % APS in HFD). The receiver mice were designated as HFD_R and APS_R, respectively. The receiver mice were fed with chow diet for consecutive eight weeks first, and then switched to HFD for another four weeks.Results:APS effectively suppressed the HFD-induced obesity formation, attenuated the hepatic steatosis, decreased hepatic TG level, improved insulin sensitivity, restored the balance of gut microbiota in HFD-fed mice by increasing the relative abundance of Bacteroidetes and Firmicutes, and reducing the abundance of Proteobacteria bacteria. Moreover, the anti-obesity effect of APS could be transferred to mice that were transplanted with intestinal bacteria from APS-fed mice in the context of HFD, but not chow diet.Conclusion:APS could inhibit the HFD-induced obesity formation, at least partially, by modulating gut microbiota. |
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