| 引用本文:党晓洁.藏红花对糖尿病大鼠视网膜神经节细胞凋亡过程中线粒体途径的影响[J].世界中医药,2016,(11):. |
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| 藏红花对糖尿病大鼠视网膜神经节细胞凋亡过程中线粒体途径的影响 |
| Effect of Saffron on Mitochondria Pathway during Apoptosis of Retinal Ganglion Cells in Diabetic Rats |
| 投稿时间：2015-11-05 |
| DOI：10.3969/j.issn.1673-7202.2016.11.048 |
| 中文关键词: 藏红花 糖尿病 视网膜神经节 细胞凋亡 线粒体 功能 |
| English Keywords:Saffron Diabetes mellitus Retinal ganglion cells Apoptosis Mitochondrial Function |
| 基金项目:国家自然科学基金项目(编号:81273902)——藏红花对改善线粒体功能抑制视网膜神经节细胞凋亡的机制研究 |
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| 中文摘要: |
| 目的:观察藏红花对糖尿病大鼠视网膜神经节细胞凋亡过程中线粒体功能的影响。方法:采用随机数字表法将72只8~10周龄健康无眼疾SD雄性大鼠随机分为正常对照组、糖尿病模型组、藏红花治疗组,每组24只。正常对照组大鼠不作任何处理。其余组采用链脲佐菌素(SYZ)25 mg/(kg·d)连续腹腔注射3 d,1次/d,建立2型糖尿病模型,以血糖≥167 mmol/L为造模成功。模型成功建立后,藏红花治疗组50 mg/kg的剂量腹腔注射藏红花素溶液,1次/d,连续3个月。并检测3组大鼠视网膜醛糖还原酶(AR)、神经细胞凋亡及视网膜中B细胞淋巴瘤/白血病-2(bcl-2)、bcl-2相关X蛋白(bcx)、细胞色素C(cyt-c)、半胱天冬酶-3(cespase-3)蛋白的表达。结果:糖尿病模型组大鼠视网膜中的AR活性明显高于正常对照组和藏红花治疗组,且差异有统计学意义(P<005);对照组RGCs层见少量TUNEL染色阳性细胞,成棕褐色染色;B:糖尿病模型组RGCs、内核层可见大量TUNEL染色阳性细胞;C:藏红花治疗组RGCs、内核层可见量TUNEL染色阳性细胞,但数量远少于糖尿病模型组;糖尿病模型组大鼠视网膜中的bcl-2、bax、cyt-c及caspase-3蛋白表达均明显低于正常对照组和藏红花治疗组,且差异有统计学意义(P<005)。结论:藏红花可通过抑制AR活性、上调凋亡抑制基因bcl-2的表达及下调凋亡促进基因bax、cyt-c和caspase-3的表达,抑制线粒体凋亡途径活化,减少糖尿病大鼠视网膜神经细胞的凋亡,发挥对糖尿病视网膜损伤的保护作用。 |
| English Summary: |
| To observe the effect of saffron on mitochondrial function of diabetic rat retinal ganglion cell apoptosis process.Methods:Using random number table method,72 SD male rats 8 ~ 10 weeks old with healthy eyes were randomly assigned to normal control group, diabetic model group, and saffron treatment group (n=24). Normally controlled rats experienced no treatment. Other rats were given streptozocin (SYZ) 25 mg / (kg / D) by intraperitoneal injection for 3 days,once daily, to establish the model of type 2 diabetes and their blood sugar would be more than or equal to 167 mmlo/L for a successful modeling. After the model was established, saffron treatment group was provided with intraperitoneal injection of crocin solution 50 mg / kg, once per day, for 3 consecutive months. And Retinal aldose reductase (AR), neural cell apoptosis and B cell lymphoma / leukemia-2 (Bcl-2), cell pigment and Bcl-2 associated X protein (BCX), cytochrome C (Cyt-C), cespase-3 protein expression were detected.Results:The AR activity in the retina of diabetic rats was significantly higher than that of the normal control group and the treatment group, and the difference was statistically significant (P<005); there was small amount of TUNEL positive cells in RCGs layers of the control group, brown staining; B: The RGCs and nuclear layer in diabetic model group had a number of TUNEL staining positive cells; C: there were TUNEL staining positive cells in RGCs and kernel layer in Saffron treatment group, but much less than the number of that of the diabetic model group; Retinal Bcl-2, Bax, Cyt-C and caspase-3 protein expression of diabetic rats were much lower than those of normal control group and treatment group, and the difference is statistically significant (P<005).Conclusion:Saffron inhibited AR activity and the activation of the mitochondrial apoptotic pathway, regulated expression of apoptosis inhibition of bcl-2 gene and apoptosis promoting gene Bax, Cyt-C and Caspase-3, reduced the apoptosis of nerve cells in the retina of diabetic rats and showed protective effect on diabetes optic omental injury. |
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