世界中医药

作者	单位
郑桂芝1，李晗1，吴焕淦1，2，马晓芃2，方臻臻1，马喆1，周次利2，施征2，刘慧荣1，2	1 上海中医药大学附属岳阳中西医结合医院，上海，200437； 2 上海中医药大学上海市针灸经络研究所，上海，200030

摘要点击次数: 971

全文下载次数: 716

中文摘要:

目的：围绕体表神经性瞬时电位感受器香草酸亚型1（Neuronal Transient Receptor Potential Vanilloid 1，nTRPV1）研究温和灸皮肤启动机制和对内脏痛模型的镇痛效果。方法：野生型C57BL/6小鼠作为主要研究对象。于8周龄时予130 μg/mL硝基苯磺酸灌肠（Three Nitrobenzene Sulfonic Acid，TNBS）诱导慢性内脏痛模型小鼠。12周龄时，温和灸组（Moxibustion，Mox）和假温和灸组（Sham Mox）的小鼠足三里穴区接受温和灸刺激，Mox的穴区皮肤温度控制在（45±1）℃，而Sham Mox控制在（37±1）℃。借用腹部撤回反射评分（Abdominal Withdrawal Reflex，AWR）作为疼痛主要观察指标。使用神经纤维素蛋白（Protein Gene Product 9.5，PGP9.5）和TRPV1免疫荧光双标，观察艾灸对于nTRPV1的影响。同时使用TRPV1阻断剂——辣椒平（Capsazepine，CPZ），分别以高、低剂量阻断体表TRPV1，观察Mox的镇痛和体表nTRPV1的表达。另外，又分别使用坐骨神经结扎和树脂毒素（Resiniferatoxin，RTX）阻断皮下nTRPV1，研究Mox的镇痛和体表nTRPV1表达。结果：相对于Sham Mox，Mox显著改善了内脏痛模型小鼠的AWR评分（P<0.05）和促进nTRPV1的表达量增加。使用CPZ阻断后，Mox未能引起AWR的改善，但低剂量的CPZ无法抑制Mox引起的nTRPV1的增多。而使用nTRPV1阻断方式后，发现体表的nTRPV1显著减少。Mox不能有效的发挥镇痛效果，而且不能促进nTRPV1的增多。结论：温和灸可能通过影响体表nTRPV1的方式，对内脏痛模型发挥相应的镇痛效果，阻断nTRPV1之后，温和灸镇痛效果消失。

English Summary:

To study the relationship between the cutaneous starting mechanism and the analgesia effect of warm moxibustion on visceral pain by the cutaneous neuronal transient receptor potential vanilloid 1 (nTRPV1). Methods:SPF wild type C57BL/6 mouse were involved in this study. At the week 8, the visceral pain was modeled by 130 μg/mL TNBS injected into the colon in SPF C57BL/6 wild type mice. During the week 12, mice in the Mox group and Sham Mox group received warm moxibustion on ST-36. The cutaneous temperature was controlled around (45±1)℃ in the Mox group while (37±1)℃ in the Sham Mox group. The analgesia effect of moxibustion was assessed by Abdominal withdrawal reflex (AWR). And nTRPV1 in the cutaneous were observed by immunofluorescence which was marked with protein gene product 9.5 (PGP9.5) and TRPV1. Low and high volume capsazepine (CPZ) inhibiting cutaneous TRPV1 activity were involved to observe the changes of analgesia effect and the nTRPV1 expression. Furthermore, sciatic nerve ligation (SNL) and resiniferatoxin (RTX) inhibiting nTRPV1 activity were used to study the differences of analgesia and nTRPV1 expression respectively. Results:Comparing with Sham Mox, Mox significantly reduced the AWR score in the visceral pain mice (P<0.05), and increased the expressions of nTRPV1. After CPZ being involved, the analgesia effect of Mox was inhibited, but low volume CPZ was not able to inhibit the promotion of nTRPV1 by moxibustion. Both SNI and RTX involved were able to inhibit the analgesia and the nTRPV1 of moxibustion. Conclusion:Warm moxibustion is able to improve the pain level of visceral pain which may be mediated by the cutaneous nTRPV1. After blocked the TRPV1 in the skin, the analgesia of warm moxibustion disappeared.

查看全文查看/发表评论下载PDF阅读器