| 引用本文:吴璐一1，顾沐恩2，朱毅3，刘慧荣3，秦文政4，黄艳3，窦传字3，袁凌松3，陆颖1，周次利3，李璟2.基于UPLC-Q/TOF MS技术的隔药饼灸合募配穴治疗UC大鼠的尿液代谢组学研究[J].世界中医药,2016,(12):. |
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| 基于UPLC-Q/TOF MS技术的隔药饼灸合募配穴治疗UC大鼠的尿液代谢组学研究 |
| Urine Metabonomic Research of Herbs-partition Moxibustion with Mactching Mu Points in UC Rats Based on UPLC-Q/TOF MS |
| 投稿时间：2016-12-07 |
| DOI：10.3969/j.issn.1673-7202.2016.12.009 |
| 中文关键词: 灸法 代谢组学 超高效液相色谱-四级杆飞行时间质谱 溃疡性结肠炎 |
| English Keywords:Moxibustion Metabonomic UPLC-Q/TOF MS Ulcerative colitis |
| 基金项目:国家重点基础研究发展计划（“973”计划）项目（编号：2009CB522900；2015CB554501）；国家自然科学基金青年项目(编号：81303033)；上海市科技启明星计划基金资助项目（编号：16QA1403400） |
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| 中文摘要: |
| 目的：运用代谢组学用超高效液相色谱-四级杆飞行时间质谱（UPLC-Q/TOF MS）技术探讨隔药饼灸合募配穴治疗溃疡性结肠炎（Ulcerative Colitis，UC）的作用机制。方法：27只SD大鼠随机分为正常组、模型组、隔药饼灸合募配穴组，采用DSS水溶液饮用法制备UC大鼠模型。隔药饼灸合募配穴组每次每穴各灸2壮，1次/d，共灸14次。治疗结束后，搜集各组尿液，UPLC-Q/TOF MS分析检测，主成分分析（PCA）分析和偏最小二乘法分析（PLS-DA）对数据进行处理，运用Metlin(http://metlin.scripps.edu/)、KEGG(http://www.kegg.jp)、HMDB(http://www.hmdb.ca)、MetaboAnalyst 3.0（http://www.metaboanalyst.ca）数据库和网站，确认潜在生物标志物，分析代谢物通路。结果：正、负离子模式下，正常组、模型组、隔药饼灸合募配穴组3组大鼠尿液在PLS-DA数据模型下分离趋势良好。隔药饼灸合募配穴可有效回调UC模型大鼠6个差异代谢物L-Cysteine（半胱氨酸）、Atraric acid、Tocopheronic acid、Dibutyl malate（二丁基苹果酸盐）、L-Tyrosine（酪氨酸）、L-Pipecolic acid（哌啶酸）表达，主要参与Phenylalanine，tyrosine and tryptophan biosynthesis（苯丙氨酸、酪氨酸和色氨酸生物合成）、Cysteine and methionine metabolism（半胱氨酸和蛋氨酸代谢）、Tyrosine metabolism（酪氨酸代谢）等氨基酸代谢通路。结论：隔药饼灸合募配穴治疗UC可能主要与氨基酸代谢有关。 |
| English Summary: |
| To explore the mechanism of herbs-partition moxibustion with the matching mu points in treating UC rats based on metabonomic with UPLC-Q/TOF MS. Methods:Sprague-Dawley rats of 27 cases were randomly divided into normal group, model group, herbs-partition moxibustion with the mactching mu points group. The UC rat model was established by drinking DSS aqueous solution. In herbs-partition moxibustion with the matching mu points group, each point moxibustion with 2 moxa cones, once per day, and lasting for 14 days. After moxibustion treatment, all groups urine was analyzed with UPLC-Q/TOF MS. The acquired data were analyzed using principal component analysis (PCA) and partial leastsquares discriminant analysis (PLS-DA).The potential biomarkers and metabolic pathways were analyzed by Metlin(http://metlin.scripps.edu/), KEGG(http://www.kegg.jp), HMDB (http://www.hmdb.ca), MetaboAnalyst 3.0(http://www.metaboanalyst.ca). Results:Under both positive ion model and negative ion model, the separation trends of 3 groups urine were good. Herbs-partition moxibustion with the matching mu points effectively reduced in 6 different metabolites expression, such as L-cysteine, atraric acid、tocopheronic acid, dibutyl malate、L-tyrosine、L-pipecolic acid, which increased in model group. All 6 different metabolites above were mainly related to amino acid metabolic pathways, such as phenylalanine, tyrosine and tryptophan biosynthesis, cysteine and methionine metabolism, tyrosine metabolism, and so on. Conclusion:The mechanism of herbs-partition moxibustion with the matching mu points in treating UC may mainly relate to amino acid metabolism. |
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