| 引用本文:黄艳1，顾沐恩2，翁志军1，张建斌3，施茵1，马喆1，窦传字1，刘慧荣1,2，吴焕淦1,2，王晓梅1，李璟2.逆灸“天枢”穴干预大鼠结肠组织基因表达谱的实验研究[J].世界中医药,2016,(12):. |
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| 逆灸“天枢”穴干预大鼠结肠组织基因表达谱的实验研究 |
| Genome-wide Gene Expressions under the Effect of Preventative Moxibustion at ST25 in Rats |
| 投稿时间：2016-12-07 |
| DOI：10.3969/j.issn.1673-7202.2016.12.010 |
| 中文关键词: 逆灸 结肠组织 基因表达谱 炎症性肠病 免疫 |
| English Keywords:Preventative moxibustion Colon tissue Gene expression profiling Inflammatory bowel disease |
| 基金项目:国家重点基础研究发展计划（”973”计划）项目（编号：2009CB522900；2015CB554501）；国家自然科学基金青年项目(编号：81503659);中国博士后科学基金面上项目(编号：2015M570380)；上海市针灸机制与穴位功能重点实验室资助（编号：14DZ2260500）；上海市重点学科资助项目（编号：S30304-A17） |
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| 中文摘要: |
| 目的：探讨逆灸“天枢”穴干预对健康大鼠结肠组织基因表达谱的影响，本文首次从转录组学的角度探讨逆灸的作用机制。方法：16只成年的雄性SD大鼠随机分成2组，正常组（NC）、正常+隔药灸组（NM），采用隔药灸天枢穴（双侧）7 d，在2%戊巴比妥钠麻醉下获取结肠组织。从大鼠的一般状况、体重、结肠组织病理学，观察隔药灸天枢穴对健康的正常大鼠的效应。每组各取3只提取总RNA，采用RNA-seq的高通量测序的方法检测结合生物信息学，分析结肠组织的mRNA表达谱。结果：与NC组比较，隔药灸不导致的健康大鼠体重降低，不影响结肠组织病理学形态观察。与NC组比较，NM组的结肠组织差异表达基因有866个，其中上调604个，下调262个。差异表达基因涉及的基因本体（GO）主要与细胞周期、免疫分子和蛋白质生物过程相关，基因通路（Pathway）主要与机体免疫和蛋白质代谢相关。结论：艾灸“天枢”穴不损伤健康成年大鼠的结肠组织的形态，或能通过影响结肠组织的差异表达基因的生物学过程及通路，调节机体免疫功能而发挥艾灸保健与养生的作用。 |
| English Summary: |
| To investigate genome-wide gene expressions of preventative moxibustion at ST25 in healthy rats. Methods:A total of 16 male Sprague-Dawley rats were randomly divided into the normal group (NC) and the normal and herb-partitioned moxibustion group (NM). Starting from day 1, the NM group received herb-partitioned moxibustion at the Tianshu points (bilateral, ST25) for 7 days. On day 8, colon tissues were collected under anesthesia with 2% pentobarbital sodium. The effect of herb-partitioned moxibustion at the ST25 in rats was observed on the general conditions, body weights, and HE staining. Three samples from each group were selected for total RNA extraction. The gene expression profile in the colon tissues was analyzed by the RNA-seq combined with bioinformatics. Results:Compared with the NC group, herb-partitioned moxibustion did not take effect on weight and histopathological observation of the colon in healthy rats. The NM group had 866 differentially expressed genes in colon tissues compared to the NC group, of which 604 were up-regulated and 262 were down-regulated. KEGG pathway analysis indicated that these genes were involved in multiple pathways, including immunity and protein metabolism. Conclusion:Herb-partitioned moxibustion at ST25 alters genome-wide gene expressions in healthy rats, and affects the biological processes and pathways. The treatment can be used as a healthcare treatment against diseases. |
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