世界中医药

目的:部分揭示气候突变对正常机体免疫功能的影响,为进一步研究极端气候变化对人体健康的影响奠定基础。方法:选用人工模拟系统,进行气温骤升、骤降干预。在干预24 h、72 h后取血,测定金黄地鼠血清MT、IL-1、IL-6及T淋巴细胞免疫相关指标。结果:1)与正常气候组比较,在气温骤升干预24 h后,血清IL-1和IL-6含量明显升高,其差异有统计学意义(P<0.01)。72 h时,血清MT、IL-1、IL-6水平下降,接近正常对照组含量;气温骤降干预后24 h,IL-1升高明显,差异有统计学意义。72 h后,血清MT、IL-1和IL-6水平均表现为明显升高,差异有统计学意义。2)在气温骤升24 h时,和正常气候组比较,CD3+、CD4+水平升高,有统计学意义(P<0.05)。CD8+表现为降低,其差异无统计学意义(P>0.05)。72 h后,CD3+和CD4+恢复至正常水平,而CD8+则进一步降低,差异有统计学意义(P<0.01);气温骤降干预时,CD3+水平升高,72 h后差异有统计学意义(P<0.05)。CD4+未出现明显变化,CD8+在干预24 h后明显降低(P<0.05),72 h时基本恢复至正常水平;在气温骤升和骤降时,CD4+/CD8+均表现为升高趋势,差异有统计学意义(P<0.05)。3)气温骤升结束24 h时,MT与IL-6、CD3+、CD8+均显著正相关,相关系数分别为0.779、0.836、0.828;气温骤升结束72 h时,MT与CD3+、CD4+、CD8+均显著正相关,相关系数分别为0.923、092、0.883、0.792;气温骤降结束24 h时,MT与CD3+、CD4+、CD8+均显著正相关,相关系数分别为0.86、0.818、0.807、0.783;气温骤降结束72 h时,MT与IL-1、IL-6、CD3+、CD4+、CD8+正相关,结论:血清MT、IL-1、IL-6、CD4+/CD8+在气温突变时水平升高,可能为气候突变影响机体免疫力的主要机制之一。MT与CD3+、CD4+、CD8+存在显著正相关,分析其机制,可能在气温骤变条件下,机体分泌MT的能力增强,血清MT含量增加,加快了T细胞的增殖速度,从而提高机体免疫力,以此对抗气温骤变带来的不利影响。

English Summary:

To illustrate the effect of sudden increase and drop in temperature on immunity in normal conditions, and to lay the foundation of a further study on effect of extreme climate change on human bodies. Methods:Manual simulation system was adopted to simulate sudden increase and drop in temperature. Blood tests were applied for measuring serum MT, IL-1, IL-6 and T-lymphocytes of golden hamsters after 24 hours and 72 hours of intervention respectively. Results:1) Compared with the normal climate group, IL-1and IL-6 levels in serum increased significantly during the 24 hours after a sharp increase in temperature (P<0.01). MT, IL-1 and IL-6 levels in serum decreased, almost the same as the normal control group content after a 72 h sharp increase in temperature； IL-1 levels increased greatly (P<0.01) after a 24 h sudden drop in temperature. MT, IL-1 and IL-6 levels in serum were significantly increased after a 72 h sudden drop in temperature. All data above showed statistical significance.2) Compared with the normal climate group, CD3+ and CD4+ levels significantly increased（P<0.05）after 24 hours of sharp increase in temperature. CD8+ level decreased, but the difference was not statistically significant（P>0.05）. After 72 h, CD3+ and CD4+ levels were normal again, but CD8+ level continued to reduce, and the difference was statistically significant. CD3+ level increased and there was a significant difference after 72 h. CD4+ Contents had no significant change. CD8+ level significantly decreased after 24 h of intervention（P<0.05）and recovered to normal level when 72 h. CD4+/CD8+ level tended to increase in sudden change of temperature（P<0.05）with statistical significance.3) After 24 h increase of temperature, MT and IL-6, CD3+ and CD8+ had positive correlation, and correlation indexes were 0.779, 0.836, 0.828; after 72 h increase of temperature, MT and CD3+,CD4+ and CD8+ had positive correlation, and correlation indexes were 0.923,092,0.883, 0.792; after 24 h decrease of temperature, MT and CD3+,CD4+ and CD8+ had positive correlation, and correlation indexes were 0.86,0.818, 0.807, 0.783; after 72 h decrease of temperature, MT and IL-1,IL-6, CD3+, CD4+ and CD8+ had positive correlation. Conclusion:Serum MT, IL-1, IL-6 and CD4+/ CD8+ levels all increased significantly in sudden change of temperature, which may be one of the main mechanisms of effect of abrupt climate changes on immunity. MT and CD3+, CD4+ and CD8+ had significantly positive correlation, which may because of more secretion of MT, increased content of MT in serum, and the multiplication rate of T-lymphocytes improved, thus the immunity was developed to cope with sudden change of temperature.

查看全文查看/发表评论下载PDF阅读器