To establish an HPLC method for simultaneous determination of 10 contents including 5 caffeoylquinic acids and 5 flavones in Chrysanthemum Flower.Methods:HPLC separation was carried out with an DiamonsilTM C18 column(4.6 mm×250 mm，5 μm)using acetonitrile-0.05% Formic acid as mobile phase at a flow rate of 1.0 mL/min.The detection wavelength was 335 nm and the column temperature was maintained at 40 ℃.Results:Good linear relationships were observed between the masses and the peak areas of the 10 contents； The average recovery rates were between 95.6 and 100.3%，RSD was between 0.66 and 1.95%.Conclusion:The content determination method can be used as an effective method for the quality control of Chrysanthemum Flower，which provides the data base for the research on the relationships between different contents of Chrysanthemum Flower.