| 引用本文:王月茹1，谢伟2.HPLC法测定药用菊花中10个主要化学成分的含量[J].世界中医药,2016,(12):. |
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| HPLC法测定药用菊花中10个主要化学成分的含量 |
| Quantification of 10 Key Contents in Chrysanthemum Flower by HPLC |
| 投稿时间：2016-03-29 |
| DOI：10.3969/j.issn.1673-7202.2016.12.066 |
| 中文关键词: 菊花药材；黄酮；咖啡酰奎宁酸；高效液相色谱法 含量测定 |
| English Keywords:Chrysanthemum Flower Flavones Caffeoylquinic acids HPLC Quantification method |
| 基金项目:陕西省中医药管理局攻关项目（编号:15-Zy033） |
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| 中文摘要: |
| 目的：建立HPLC法测定菊花药材中10个主要化学成分的含量，包括5个咖啡酰奎宁酸类成分和5个黄酮类成分。方法：采用DiamonsilTM C18(4.6 mm×250 mm，5 μm)色谱柱，流动相为乙腈-0.05%甲酸，流速1.0 mL/min，检测波长335 nm，柱温40 ℃。结果：10个化合物在一定范围内与峰面积呈良好的线性关系，r值均大于0.999 5；平均加样回收率(n=6)范围为95.6%～100.3%，RSD为0.66%～1.95%。结论：本文所建立含量测定方法可作为中药菊花的质量控制的有效方法，为明确不同品种菊花的基原关系和品种鉴定研究提供了数据基础。 |
| English Summary: |
| To establish an HPLC method for simultaneous determination of 10 contents including 5 caffeoylquinic acids and 5 flavones in Chrysanthemum Flower.Methods:HPLC separation was carried out with an DiamonsilTM C18 column(4.6 mm×250 mm，5 μm)using acetonitrile-0.05% Formic acid as mobile phase at a flow rate of 1.0 mL/min.The detection wavelength was 335 nm and the column temperature was maintained at 40 ℃.Results:Good linear relationships were observed between the masses and the peak areas of the 10 contents； The average recovery rates were between 95.6 and 100.3%，RSD was between 0.66 and 1.95%.Conclusion:The content determination method can be used as an effective method for the quality control of Chrysanthemum Flower，which provides the data base for the research on the relationships between different contents of Chrysanthemum Flower. |
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