| 引用本文:董洁，冀娇娇，王加利，袁将，王贝贝，赵爽，郑尊善，刘永刚.维吾尔族药阿纳其根中绿原酸的含量测定[J].世界中医药,2016,(12):. |
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| 维吾尔族药阿纳其根中绿原酸的含量测定 |
| Method Study for Determination of Chlorogenic Acid in Anacyclus pyrethrum DC.of Uygur Medicine by RP-HPLC |
| 投稿时间：2015-09-20 |
| DOI：10.3969/j.issn.1673-7202.2016.12.067 |
| 中文关键词: 维药 阿纳其根 HPLC 绿原酸 含量测定 |
| English Keywords:Uygur Medicine Anaycclus pyerhturm.DC. HPLC Chlorogenic acid Content determination |
| 基金项目:新疆维吾尔自治区自然科学基金项目（编号：201318101-5) |
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| 中文摘要: |
| 目的：建立测定维吾尔族药阿纳其根中绿原酸含量的高效液相色谱法（HPLC）。方法：色谱柱为Agilent 5 TC-C18柱(5 μm，4.6 mm×150 mm)，以乙腈-0.4%磷酸水（13:87，V/V）为流动相，流速为0.8 mL·min-1，紫外检测波长为327 nm。结果：绿原酸在质量为0.0484 mg～0.968 mg范围内呈良好线性关系，回归方程：Y=2296316.8X-1369.4（r=0.9999），平均回收率为100.20%，RSD=2.75%。结论：该方法简便、灵敏、重现性好，可用于阿纳其根中绿原酸的含量测定。 |
| English Summary: |
| A high performance liquid chromatography method（HPLC)was established to determine chlorogenic acid in the root of Anaycclus pyerhturm.DC.Methods:HPLC ana1ysis was actualized on an Agilent 5 TC-C18(5 μm，4.6 mm×150 mm)column with mobile phase of acetonitrile-0.4% phosphoric acid solution(13:87，V/V)，flow rate was 0.8 mL·min-1，detection wavelength was 327 nm.Results:The calibration curve was a good linear with a range of 0.0484~0.968 mg·for chlorogenic acid(Y=2296316.8X-1369.4，r=0.9999).The average recovery rate for chlorogenic acid was 100.20%，RSD=2.75%.Conclusion:This method is simple，sensitive，reproducible，which is suitable for the quality control of the root of Anaycclus pyerhturm.DC. |
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