A high performance liquid chromatography method(HPLC)was established to determine chlorogenic acid in the root of Anaycclus pyerhturm.DC.Methods:HPLC ana1ysis was actualized on an Agilent 5 TC-C18(5 μm,4.6 mm×150 mm)column with mobile phase of acetonitrile-0.4% phosphoric acid solution(13:87,V/V),flow rate was 0.8 mL·min-1,detection wavelength was 327 nm.Results:The calibration curve was a good linear with a range of 0.0484~0.968 mg·for chlorogenic acid(Y=2296316.8X-1369.4,r=0.9999).The average recovery rate for chlorogenic acid was 100.20%,RSD=2.75%.Conclusion:This method is simple,sensitive,reproducible,which is suitable for the quality control of the root of Anaycclus pyerhturm.DC.