| 引用本文:黄为钧1，王世东1，赵进喜1，傅强1，宫晴1，张华1，吴文静2，申子龙3，贾旭4，张雨婷4.益气活血散结法对糖尿病肾病大鼠肾组织中MCP-1、TGF-β的影响[J].世界中医药,2017,(01):. |
|
| 益气活血散结法对糖尿病肾病大鼠肾组织中MCP-1、TGF-β的影响 |
| Effects of Qi-tonifying Blood-activating and Mass-dissipating Method on Expressions of MCP-1 And TGF-β in Renal Tissue of Rats with Diabetic Nephropathy |
| 投稿时间：2016-12-20 |
| DOI：10.3969/j.issn.1673-7202.2017.01.004 |
| 中文关键词: MCP-1 TGF-β 益气活血散结法 糖尿病肾病 |
| English Keywords:MCP-1 TGF-β Qi-tonifying blood-activating and mass-dissipating method diabetic nephropathy |
| 基金项目:国家自然科学基金项目（编号：81473664） |
|
| 摘要点击次数: 1214 |
| 全文下载次数: 1134 |
| 中文摘要: |
| 目的：通过动物实验，基于免疫炎性反应因子MCP-1、TGF-β，探索益气活血散结法改善肾小球硬化的分子机制。方法：24只GK大鼠，随机分为模型组、假手术组、益气活血散结组。通过高脂高能量喂养加单肾切除术复制糖尿病肾病模型。检测各组大鼠的24 h尿蛋白，观察大鼠在干预后肾脏组织的病理改变，同时采用Western blotting以及免疫组化法检测肾脏组织的MCP-1和TGF-β的表达情况，采用RT-PCR法检测MCP-1mRNA以及TGF-βmRNA的表达水平。结果：PASM染色和Masson染色显示：益气活血散结组阳性率明显低于模型组（P<0.01）。益气活血散结组中24 h尿蛋白在药物干预后明显减少（P<0.01）。Western blotting、免疫组化结果均显示：益气活血散结组MCP-1（P<0.05）、TGF-β（P<0.01）的表达程度明显低于模型组。同样，RT-PCR结果也显示:MCP-1mRNA（P<0.01）、TGF-βmRNA（P<0.05）水平在益气活血散结组明显低于模型组。结论：益气活血散结法可以有效地减少24 h尿蛋白定量、延缓肾小球纤维化，其机制可能与抑制肾脏组织中MCP-1和TGF-β的表达有关。 |
| English Summary: |
| To study the mechanism of Qi-tonifying blood-activating and mass-dissipating method in treating diabetic nephropathy based on MCP-1 and TGF-β through animal experiment.Methods:A total of 24 GK rats were randomly divided into model group，sham-operated group and Qi-tonifying blood-activating and mass-dissipating group.Each group had 8 rats.The DN model was established by feeding with high-fat diet and unilateral nephrectomy.24-hour urine protein was detected and the renal pathological changes were observed.Expressions of MCP-1 and TGF-β in the renal tissue were detected by Western blotting and immunohistochemistry，while RT-PCR was used to detect the expression of MCP-1mRNA and TGF-β mRNA.Results:Results of PASM staining and Masson staining show that the positive rate of Qi-tonifying blood-activating and mass-dissipating group was significantly lower than that of model group(P<0.01).The 24-hour urinary protein in Qi-tonifying blood-activating and mass-dissipating group decreased significantly after drug intervention（P<0.01).Results of Western blotting and immunohistochemistry showed that expression levels of MCP-1(P<0.05) and TGF-β(P<0.01) were lower in Qi-tonifying blood-activating and mass-dissipating group than those in model group.Results of RT-qPCR showed that expression levels of MCP-1 mRNA(P<0.01) and TGF-β mRNA(P<0.05) in Qi-tonifying blood-activating and mass-dissipating group were significantly lower than those in model group.Conclusion:The treatment of diabetic nephropathy with Qi-tonifying blood-activating and mass-dissipating method can decrease the proteinuria and delay the glomerular fibrosis.The molecular mechanism may be related to the inhibition of MCP-1 and TGF-β. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
