Effects of Qi-tonifying Blood-activating and Mass-dissipating Method on Expressions of MCP-1 And TGF-β in Renal Tissue of Rats with Diabetic Nephropathy
To study the mechanism of Qi-tonifying blood-activating and mass-dissipating method in treating diabetic nephropathy based on MCP-1 and TGF-β through animal experiment.Methods:A total of 24 GK rats were randomly divided into model group,sham-operated group and Qi-tonifying blood-activating and mass-dissipating group.Each group had 8 rats.The DN model was established by feeding with high-fat diet and unilateral nephrectomy.24-hour urine protein was detected and the renal pathological changes were observed.Expressions of MCP-1 and TGF-β in the renal tissue were detected by Western blotting and immunohistochemistry,while RT-PCR was used to detect the expression of MCP-1mRNA and TGF-β mRNA.Results:Results of PASM staining and Masson staining show that the positive rate of Qi-tonifying blood-activating and mass-dissipating group was significantly lower than that of model group(P<0.01).The 24-hour urinary protein in Qi-tonifying blood-activating and mass-dissipating group decreased significantly after drug intervention(P<0.01).Results of Western blotting and immunohistochemistry showed that expression levels of MCP-1(P<0.05) and TGF-β(P<0.01) were lower in Qi-tonifying blood-activating and mass-dissipating group than those in model group.Results of RT-qPCR showed that expression levels of MCP-1 mRNA(P<0.01) and TGF-β mRNA(P<0.05) in Qi-tonifying blood-activating and mass-dissipating group were significantly lower than those in model group.Conclusion:The treatment of diabetic nephropathy with Qi-tonifying blood-activating and mass-dissipating method can decrease the proteinuria and delay the glomerular fibrosis.The molecular mechanism may be related to the inhibition of MCP-1 and TGF-β.