世界中医药

目的：探讨中药利湿活血方对高尿酸血症模型大鼠血尿酸及其抗氧化能力的影响。方法：将雄性SD大鼠随机分成空白组、模型组、四妙丸组、苯溴马隆组及利湿活血方（7.74 g/kg、3.87 g/kg、1.935 g/kg）组，共7组。除空白组，其余各组以酵母膏、腺嘌呤连续灌胃14 d，制备高尿酸血症模型。验模成功后，各给药组灌胃给予相应药物，空白组和模型组给予等体积蒸馏水，连续14 d。末次给药后，测定大鼠血清中血尿酸（UA）、丙二醛（MDA）含量,活性氧簇（ROS）水平及过氧化物歧化酶（SOD）、总抗氧化能力（T-AOC）、谷胱甘肽过氧化物酶（GSH-PX）、过氧化氢酶（CAT）的活性。结果：1）与空白组比较，模型组动物血清UA含量升高，差异有统计学意义（P<0.001）；ROS水平升高，差异有统计学意义（P<0.001）；MDA含量显著升高（P<0.01）；SOD、T-AOC活性明显降低，差异有统计学意义（P<0.001）。2）与模型组比较，四妙丸组、苯溴马隆组及利湿活血方7.74 g/kg、3.87 g/kg组大鼠血清UA含量明显降低，苯溴马隆组、利湿活血方7.74 g/kg组差异有统计学意义（P<0.001、P<0.01)；苯溴马隆组、利湿活血方7.74 g/kg组MDA含量降低，差异有统计学意义（P<0.05）；除四妙丸组外，各给药组SOD活性较模型组均显著升高（P<0.001）；各给药组ROS均降低，苯溴马隆组差异有统计学意义（P<0.001），利湿活血方7.74 g/kg、3.87 g/kg组差异有统计学意义（P<0.01）；四妙丸组、苯溴马隆组及利湿活血方7.74 g/kg、3.87 g/kg组大鼠血清T-AOC活性明显升高，苯溴马隆组差异有统计学意义（P<0.001），利湿活血方7.74 g/kg组差异有统计学意义（P<0.01）。结论：中药利湿活血方从湿热壅盛、瘀血阻滞的病机出发，以清热利湿、化瘀散结为治法，在降低高尿酸血症大鼠血清尿酸的同时，还能增强高尿酸血症大鼠的抗氧化和清除氧自由基的能力，减少脂质过氧化，达到较好的治疗效果。

English Summary:

To study the effects of Lishi Huoxue formula (LSHX) on blood uric acid and antioxidant capacity of hyperuriceamia rats. Methods:Male SD rats were randomly devided into 7 groups:blank group, model group, Simiao pill group, Benzbromarone group, and LSHX groups (7.74 g/kg，3.87 g/kg and 1.935 g/kg). Except the blank group, the rest groups were administered with yeast extract and adeline intragastrically for two weeks to build hyperuriceamia models. After that, drugs were administered to the rats in corresponding group, and the blank group and model group were given distilled water for 14 days. Then the levels of UA, MDA, SOD, ROS, T-AOC, GSH-PX, CAT in serum were detected. Results:1) Compared with the blank group, serum UA content of the model group increased greatly with significant difference (P<0.001). ROS level increased too (P<0.001), and also with the content of MDA (P<0.01). On the other hand, the activity of SOD and T-AOC were obviously lower with significant difference (P<0.001). 2) Compared with the model group, levels of serum UA in Simiao pill group, Benzbromarone group, LSHX 7.74 g/kg and LSHX 3.87 g/kg group were obviously lower, and the difference of Benzbromarone group and LSHX 7.74 g/kg group was statistically significant (P<0.001, P<0.01). The MDA content of Benzbromarone group and LSHX 7.74 g/kg group were significantly lower than that of the model group, with significant difference (P<0.05). Except Simiaowan group, the SOD activity of the other groups was obviously higher, with significant difference (P<0.001). Besides, the ROS level of each drug group decreased, Benzbromarone group (P<0.001), LSHX 7.74 g/kg and 3.87 g/kg groups (P<0.01) dropped with great significance. Serum T-AOC activity of Simiao pill group, Benzbromarone group and LSHX 7.74 g/kg and LSHX 3.87 g/kg was obviously higher than that of the model group, and the difference between Benzbromarone group and the model group was statistically significant (P<0.001), also between LSHX 7.74 g/kg and LSHX 3.87 g/kg groups and model group (P<0.01, P<0.05). Conclusion:The Chinese herbal compounds LSHX can reduce the hyperuriceamia rat′s blood uric acid, and improve antioxidant and free radical scavenging capacities in hyperuricemia rats by clearing dampness and heat and removing stasis and stagnation. The Chinese herbal compound LSHX has a great therapeutic effect.

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