| 引用本文:陈汉裕1，陈凤丽1，林赞檬1，杨忠奇2.安神补心胶囊对小鼠大脑内神经递质影响[J].世界中医药,2017,(01):. |
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| 安神补心胶囊对小鼠大脑内神经递质影响 |
| Effects of Anshen Buxin Capsule on Neurotransmitter in Cerebral Tissue of Mice |
| 投稿时间：2016-04-03 |
| DOI：10.3969/j.issn.1673-7202.2017.01.035 |
| 中文关键词: 安神补心胶囊 神经递质 睡眠 机制 |
| English Keywords:Anshen Buxin capsule Neurotransmitter Sleep Mechanism |
| 基金项目:国家级大学生创新创业训练计划项目（编号：201410572008） |
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| 中文摘要: |
| 目的：观察安神补心胶囊对小鼠大脑神经递质5-羟色胺(5-HT)及γ-氨基丁酸（GABA)含量的影响，初步探讨安神补心胶囊改善睡眠作用机制，为临床研究提供实验理论依据。方法：将昆明小鼠随机为空白对照组、地西泮对照组、安神补心胶囊组，分别灌相应药物：0.9%氯化钠注射液、地西泮水溶液（0.1 mg/mL）及安神补心胶囊水溶液(0.1 g药丸/mL)，每天灌胃给药1次(0.1 mL/10 g)，连续灌胃8 d，末次给药后，用酶联免疫吸附测定法测定小鼠大脑5-HT及GABA含量。结果：空白对照组、地西泮对照组、安神补心胶囊组5-HT含量分别为(604.56±31.35)ng/mL、(468.42±30.33)ng/mL、(518.21±31.20)ng/mL；GABA含量分别为(10.77±1.04)μmol/L、(14.58±0.75)μmol/L(12.07±0.88)μmol/L。与空白对照组相比，安神补心胶囊组能明显降低小鼠大脑5-HT含量及提高小鼠大脑GABA含量，差异有统计学意义（P<0.05），与地西泮对照组比较，差异亦具有统计学意义（P<0.05）。结论：安神补心胶囊具有良好的改善睡眠作用，其作用机制可能与抑制减少大脑5-HT的合成及刺激增加大脑GABA的分泌有关。 |
| English Summary: |
| To study the effect of Anshen Buxin capsule on serotonin ( 5-HT) and γ-aminobutyric acid content in cerebral tissue of mice, so as to make a preliminary discussion on its mechanism of improving sleep and then experimental theory for clinical study. Methods:Kunming mice were randomly divided into 3 groups, and each group was filled the corresponding drugs intragastrically:normal saline,aqueous solution of diazepam (0.1 mg/mL) and Anshen Buxin capsule (0.1 g/mL). All of those were given once a day (0.1 mL/10 g) and lasted for 8 days. After the last day of drug administration,use ELASA method to detect the content of 5-HT and GABA in mice brain serum.Results:The contents of 5-HT and γ-aminobutyric acid in normal, diazepam and Anshen Buxin capsule group were (604.56±31.35)ng/mL, (10.77±1.04)μmol/L, (468.42±30.33)ng/mL, (14.58±0.75)μmol/L; (518.21±31.20)ng/mL, and (12.07±0.88)μmol/L. Compared with normal saline group, the 5-HT content of Anshen Buxin capsule group reduced and GABA content in cerebral tissue of mice increased with significant differences (P<0.05). Besides, compared with diazepam control group, significant difference also exists (P<0.05). Conclusion:Anshen Buxin capsule had a sound effect in improving sleep quality, and its mechanism may be related to inhibit the production of 5-HT and stimulate GABA secretion in brain. |
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