| 引用本文:尹平，陈泽钦，韩栩珂，徐世芬.电针对抑郁大鼠行为学及海马β-CaMKⅡ蛋白的影响[J].世界中医药,2017,(02):. |
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| 电针对抑郁大鼠行为学及海马β-CaMKⅡ蛋白的影响 |
| Effect of Electroacupuncture on Depressive-like Behavior and β-CaMKⅡ of Habenula in the Depression Rats Model |
| 投稿时间：2017-02-18 |
| DOI：10.3969/j.issn.1673-7202.2017.02.037 |
| 中文关键词: 电针 抑郁症 缰核 β-CaMKⅡ |
| English Keywords:Electroacupuncture Depression Hippocampus β-CaMKⅡ |
| 基金项目:上海市卫生和计划生育委员会项目（编号：ZY3-CCCX-3-3022）；上海市教委科研创新重点项目（编号：2014zz116） |
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| 中文摘要: |
| 目的：观察针刺对抑郁大鼠的行为学改变及作用机制。方法：将18只雄性WKY大鼠随机分为电针组（EA）、假针组（sham EA）、模型组（model）。Wistar雄性大鼠6只设为正常组（normal）。21 d后通过糖水使用、强迫游泳实验对大鼠进行行为学评价，用免疫荧光法检测大鼠海马中β-CaMKⅡ表达。结果：与正常组比较，模型组海马β-CaMKⅡ表达和蔗糖水摄入比均明显下降（P<0.05），强迫游泳的不动时间增加（P<0.05）。与假针组比较，电针组海马β-CaMKⅡ表达和蔗糖水摄入比均明显增加（P<0.05），强迫游泳的不动时间减少（P<0.05）。结论：电针对WKY抑郁模型大鼠的行为学核心症状有明显的改善作用，明显增加海马中β-CaMKⅡ的表达，这可能是电针通过影响海马的β-CaMKⅡ从而治疗抑郁症的机制之一。 |
| English Summary: |
| To observe the effects of electroacupuncture(EA) on depressive-like behavior and its mechanism.Methods:Eighteen male WKY rats were randomly divided into EA,Sham,and model groups.Six Wistar rats were divided into normal group.The behaviors were evaluated by sucrose preference test and force swimming test after 21 days,and the expression of β-CaMKⅡ was detected by immunofluorescence.Results:Compared with the control group,the level of β-CaMKⅡ expression of hippocampus in the model group decreased significantly along with Sucrose preference test(P<0.05).Meanwhile,immobile time of force swimming test increased significantly (P<0.05).Compared with sham group,EA group increased significantly in the β-CaMKⅡ expression of hippocampus and sucrose preference test (P<0.05)，and decreased significantly in immobile time of force swimming test(P<0.05).Conclusion:Electroacupuncture can improve the WKY rats′ depressive-like behaviors and the expression of β-CaMKⅡ protein in hippocampus.This might be one of the mechanisms of Electroacupuncture on depression which is mediated by hippocampus. |
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